Cell Mechanics and Protein Mobility during Neoplastic Transformation

肿瘤转化过程中的细胞力学和蛋白质迁移率

• 批准号: 1106105
• 负责人: Keith Bonin
• 金额: $ 40万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1106105&HistoricalAwards=false
• 关键词: Cell; Mechanics; Protein; Mobility; during

The primary research objective of this grant is to advance the fundamental understanding of the different physical and mechanical properties of cells as they advance through different stages of neoplastic transformation from normal to the metastatic state. Since recent reports indicate there is significant ambiguity about how these properties change for different cancer cells, the investigators plan to measure these properties for a single line of cells, and to determine whether the changes vary for different cellular components: i.e. whether the change in physical properties is due to a change in the cytoskeleton, the cell membrane, the cytoplasm, or a combination of these elements. Measurements using a wide array of techniques from physics and cellular biology will be applied to the different cellular structures. In addition, the investigators will create a biophysical computer model that brings together all measurements to account for the observed variations in physical properties at each state of neoplastic transformation.This work will help to establish and disseminate new protocols and techniques for determining the differences in physical properties between cancerous and noncancerous cells. A team of physicists and biologists will train graduate and undergraduate students to work in this highly interdisciplinary field. Particular emphasis will be given to the education of minorities, through the connections to the MARC U* STAR programs of NC A&T University and Winston-Salem State University.

该补助金的主要研究目标是促进对细胞不同物理和机械特性的基本理解，因为它们通过从正常到转移状态的肿瘤转化的不同阶段而发展。 由于最近的报告表明，对于不同的癌细胞，这些特性如何变化存在显着的模糊性，研究人员计划测量单个细胞系的这些特性，并确定不同细胞成分的变化是否不同：即物理特性的变化是否是由于细胞骨架，细胞膜，细胞质或这些元素的组合的变化。 使用物理学和细胞生物学的各种技术进行测量将应用于不同的细胞结构。 此外，研究人员还将建立一个生物物理计算机模型，将所有测量结果汇集在一起，以解释在肿瘤转化的每个状态下观察到的物理特性变化。这项工作将有助于建立和推广新的协议和技术，以确定癌细胞和非癌细胞之间的物理特性差异。 一个由物理学家和生物学家组成的团队将培训研究生和本科生在这个高度跨学科的领域工作。 将通过与NC A T大学和州立大学温斯顿-塞勒姆的MARC U* 星星方案的联系，特别重视少数民族的教育。