SBIR Phase I: SNP Discovery to Identify Candidate Genes for Association Genetics in "Pongamia pinnata", a High-Potential Biodiesel Feedstock

SBIR 第一阶段：发现 SNP，以鉴定高潜力生物柴油原料“水黄皮”中关联遗传学的候选基因

• 批准号: 1112926
• 负责人: Claire Kinlaw
• 金额: $ 14.99万
• 依托单位: TerViva BioEnergy Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1112926&HistoricalAwards=false
• 关键词: SBIR; Phase; SNP; Discovery; Identify

This Small Business Innovation Research (SBIR) Phase I project will test the feasibility of a ?whole transcriptome? shotgun DNA sequencing approach to identify candidate alleles that can be associated with agronomically important traits in Pongamia pinnata, a leguminous tree that produces large quantities of seed oil suitable for biodiesel refining. This approach looks simultaneously for specific DNA changes (SNPs) among large numbers of expressed gene variants, alleles, that may correlate with physical traits. Specific DNA markers of these alleles will permit the tracking of multiple valuable traits within breeding populations and accelerate the development of commercial stocks of Pongamia, This remarkable but undomesticated, biofuel feedstock tree grows on marginal land unsuited for food production, fixes nitrogen in association with rhizobium, and requires low levels of water. The broader/commercial impacts of this research are that commercialization of Pongamia will proceed more readily if based solidly upon research and breeding to produce a phenotypically stable and predictable crop. The major issue for the biodiesel refining industry remains the lack of a scalable, reliable, cost effective feedstock of raw oil, not the lack of refining capacity. Pongamia provides owners of marginal land with just such a crop that can yield oil for the biofuel industry at a profit to land owners and refiners, thus offering a real market solution to renewable transportation fuel.

这个小型企业创新研究 (SBIR) 第一阶段项目将测试“整个转录组”的可行性。鸟枪法 DNA 测序方法可识别与水黄皮 (Pongamia pinnata) 农艺重要性状相关的候选等位基因，水黄皮 (Pongamia pinnata) 是一种豆科树，可生产大量适合生物柴油精炼的种子油。这种方法同时寻找大量表达基因变体、等位基因中的特定 DNA 变化 (SNP)，这些变化可能与身体特征相关。这些等位基因的特定 DNA 标记将允许追踪繁殖种群内的多个有价值的性状，并加速水黄皮商业种群的发展。这种非凡但未驯化的生物燃料原料树生长在不适合粮食生产的边际土地上，与根瘤菌结合固氮，并且需要少量的水。这项研究的更广泛/商业影响是，如果坚实地基于研究和育种以生产表型稳定且可预测的作物，水黄蜂的商业化将更容易进行。 生物柴油精炼行业的主要问题仍然是缺乏可扩展、可靠、具有成本效益的原油原料，而不是缺乏精炼能力。 Pongamia 为边际土地所有者提供了这样一种作物，可以为生物燃料行业生产石油，为土地所有者和炼油商带来利润，从而为可再生运输燃料提供真正的市场解决方案。