SBIR Phase I: Inexpensive and Effecient System for Signal Amplification

SBIR 第一阶段：廉价且高效的信号放大系统

• 批准号: 1113377
• 负责人: Alexey Zdanovsky
• 金额: $ 15万
• 依托单位: Alpha Universe LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1113377&HistoricalAwards=false
• 关键词: SBIR; Phase; Inexpensive; Effecient; System

This Small Business Innovation Research (SBIR) Phase I project has the goal of creating a signal amplification system that, while being inexpensive and simple to use, can be combined with antibodies or other probe molecules, and can increase detection limits of conventional ELISAs by 100- to 1000-fold, or even higher. To prove the concept, it is proposed to apply this system to rabbit antibodies and demonstrate that, in combination with rabbit antibodies raised against botulinum neurotoxin A, it will be able to provide sensitivity of detection similar to that of immuno-PCR, but at a fraction of the cost.The broader impact/commercial potential of this research is expected in the areas of basic research, biodefense, and medicine. This system will allow very sensitive and inexpensive detection of antigens of choice. For those involved in basic research, the proposed immunoassay will allow a significantly more detailed analysis of biological processes, possibly even opening the doors to monitoring those processes and molecules previously undetectable. In biodefense and medical diagnostics, increased sensitivity will mean an increased security by allowing earlier detection of harmful pathogens, as well as pathological change.

这个小型企业创新研究(SBIR)第一阶段项目的目标是创建一种信号放大系统，该系统成本低廉、使用简单，可以与抗体或其他探针分子结合，并可以将传统ELISA的检测极限提高100到1000倍，甚至更高。为了证明这一概念，建议将该系统应用于兔抗体，并证明与兔抗肉毒神经毒素A抗体相结合，它将能够提供与免疫-PCR相似的检测灵敏度，但成本仅为其一小部分。该研究有望在基础研究、生物防御和医学领域产生更广泛的影响/商业潜力。该系统将允许非常灵敏和廉价的选择抗原检测。对于那些参与基础研究的人来说，拟议的免疫分析将允许对生物过程进行更详细的分析，甚至可能打开监测那些以前无法检测到的过程和分子的大门。在生物防御和医学诊断中，更高的灵敏度将意味着更高的安全性，因为它允许更早地检测有害病原体和病变。