SBIR Phase I: Phage detectors for bacterial pathogens

该小型企业创新研究（SBIR）第一阶段项目的目标是创建一个系统，该系统虽然价格低廉且易于使用，但将能够分析样品中是否存在活病原菌，并确定其物种，以及在短短几个小时内对抗生素的敏感性。作为检测器，该系统将利用修饰的噬菌体。为了证明这一概念，建议创建针对弯曲杆菌的系统的第一个检测器，弯曲杆菌正在成为全球人类胃肠道感染的最重要原因之一。这项研究的更广泛的影响预计在生物防御领域和医学，其中一个灵活的检测系统将允许同时多参数监测环境或食品和水供应的安全，以及使医生能够选择适当的抗生素在细菌感染的早期阶段，从而提高治疗效率和减少耐药细菌的传播。

This Small Business Innovation Research (SBIR) Phase I project has a goal of creating a system that, while being inexpensive and simple to use, will be able to analyze samples for the presence of a live pathogenic bacteria and determine their species, as well as sensitivities to antibiotics within just a few hours. As detectors, this system will utilize modified bacteriophages. To prove the concept, it is proposed to create the first detector of the system targeted at Campylobacters, which are emerging as one of the most significant causes of human gastrointestinal infections worldwide. The broader impacts of this research are expected in the biodefense area and medicine where a flexible detection system will allow simultaneous multiparameter monitoring of the environment or food and water supplies for their safety, as well as enable physicians to choose the appropriate antibiotic at very early stages of bacterial infection, thus increasing the efficiency of treatment and reducing the spread of antibiotic-resistant bacteria.