SBIR Phase I: Phage detectors for bacterial pathogens

这个小型企业创新研究(SBIR)第一阶段项目的目标是创建一种系统，该系统在成本低廉、使用简单的同时，能够在几个小时内分析样本中是否存在活的病原菌，并确定它们的种类，以及对抗生素的敏感性。作为探测器，该系统将使用改良的噬菌体。为了证明这一概念，有人提议创建该系统的第一个针对弯曲杆菌的检测器，弯曲杆菌正成为全球人类胃肠道感染的最重要原因之一。这项研究预计将在生物防御领域和医药领域产生更广泛的影响，在这些领域，灵活的检测系统将允许同时对环境或食品和水供应进行多参数监测，以确保它们的安全，并使医生能够在细菌感染的非常早期阶段选择适当的抗生素，从而提高治疗效率并减少抗生素耐药性细菌的传播。

This Small Business Innovation Research (SBIR) Phase I project has a goal of creating a system that, while being inexpensive and simple to use, will be able to analyze samples for the presence of a live pathogenic bacteria and determine their species, as well as sensitivities to antibiotics within just a few hours. As detectors, this system will utilize modified bacteriophages. To prove the concept, it is proposed to create the first detector of the system targeted at Campylobacters, which are emerging as one of the most significant causes of human gastrointestinal infections worldwide. The broader impacts of this research are expected in the biodefense area and medicine where a flexible detection system will allow simultaneous multiparameter monitoring of the environment or food and water supplies for their safety, as well as enable physicians to choose the appropriate antibiotic at very early stages of bacterial infection, thus increasing the efficiency of treatment and reducing the spread of antibiotic-resistant bacteria.