Biochemical Mechanism of Regulation of Phytochrome Interacting Factor 1 by Light

光调控光敏色素相互作用因子1的生化机制

• 批准号: 1120946
• 负责人: Enamul Huq
• 金额: $ 55.31万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-11-01 至 2015-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1120946&HistoricalAwards=false
• 关键词: Biochemical; Mechanism; Regulation; Phytochrome; Interacting

Light is a critical factor regulating plant growth and development. For example, red and far-red light regulate important processes like seed germination and greening. The phytochrome family of photoreceptors monitors the red (R)/far-red (FR) light and initiate these responses. This project will elucidate poorly understood biochemical mechanisms of phytochrome action. Degradation of critical regulatory proteins (transcription factors) both in the dark and light has been shown to play a central role in photomorphogenesis. In general, two classes of light signaling factors are regulated by degradation: Positive regulators of photomorphogenesis such as HY5, LAF1 and HFR1 are degraded in the dark and negative regulators called Phytochrome Interacting Factors (PIFs) are degraded in response to light. Although dark-induced degradation of positive regulators has been thoroughly investigated, light-induced degradation of PIFs is less well understood. This project will use biochemical, molecular and genetic approaches to elucidate early events in the light-induced degradation PIF1 under blue, red and far-red light conditions. Light-induced degradation of PIF1 relieves the negative regulation by PIF1 to promote seed germination and greening, and inhibit hypocotyl growth. The broader impacts of the project include fundamental advances, human resource development, and training. Since PIF1 is a primary phytochrome-signaling partner whose stability is regulated by phytochromes, the project will provide fundamental information on these important processes. Moreover, as transcription factors are critical regulators of all developmental processes, understanding the mechanism of PIF1 regulation will be enabling for agricultural biotechnology and biomass production. Project results and the research tools developed will be distributed through publications, conference presentations, and website postings as well as directly from the laboratory. This project will provide needed training for undergraduate, graduate and postdoctoral students in modern functional genomics and molecular genetics. Finally, aspects of the project will be actively integrated into undergraduate and graduate classroom teaching.

光是调节植物生长发育的关键因素。例如，红光和远红光调节着种子发芽和绿化等重要过程。光敏色素家族的光感受器监测红色(R)/远红色（FR）光并启动这些反应。该项目将阐明尚不清楚的光敏色素作用的生化机制。在黑暗和光照条件下，关键调节蛋白（转录因子）的降解已被证明在光形态发生中起着核心作用。一般来说，两类光信号因子受降解调节：光形态发生的正调节因子如HY5、LAF1和HFR1在黑暗中降解，而被称为光敏色素相互作用因子（pif）的负调节因子在光照下降解。虽然暗诱导的正调节因子降解已被彻底研究，但光诱导的pif降解尚不清楚。该项目将使用生化、分子和遗传学方法来阐明在蓝光、红光和远红光条件下光诱导降解PIF1的早期事件。光诱导PIF1的降解解除了PIF1的负调控，促进种子萌发和绿化，抑制下胚轴生长。该项目的更广泛影响包括基础进步、人力资源开发和培训。由于PIF1是光敏色素信号的主要伙伴，其稳定性受光敏色素的调节，因此该项目将提供有关这些重要过程的基础信息。此外，由于转录因子是所有发育过程的关键调控因子，了解PIF1调控机制将有助于农业生物技术和生物质生产。项目成果和开发的研究工具将通过出版物、会议报告和网站发布以及直接从实验室发布。本项目将为现代功能基因组学和分子遗传学的本科生、研究生和博士后提供必要的培训。最后，将项目的各个方面积极融入到本科和研究生的课堂教学中。