The role of the RIM-binding protein active zone scaffold in regulating neurotransmitter release at diverse central synapses (A05)

RIM结合蛋白活性区支架在调节各种中枢突触神经递质释放中的作用（A05）

• 批准号: 202190057
• 金额: --
• 依托单位: Neurowissenschaftliches Forschungszentrum (CCM)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/202190057?language=en
• 关键词: role; RIM; binding; protein; active

How the protein scaffolds that mediates synaptic vesicle exocytosis contributes to the brain wide diversity of synaptic function is largely unknown. We will examine the role of RIM-binding protein in defining the molecular architecture and function in two central murine synapses with known differences in function. We will characterize the RIM-BP2 interactors and their functional implication using biochemistry, in vivo mutagenesis and microscopy. We will examine putative mechanisms for alternative synapse specific scaffold formations such as variations in expression of isoforms of RIM-BP2 and it’s interaction partners, or variation of protein levels. The proposed experiments promise to reveal scaffolding principles that contribute to the functional architecture of the active zone and open up novel understanding of the biology that underlies diversity of synaptic coding.

介导突触囊泡胞吐的蛋白质支架如何有助于大脑突触功能的广泛多样性在很大程度上是未知的。我们将研究RIM结合蛋白在定义两个已知功能差异的中枢小鼠突触的分子结构和功能中的作用。我们将使用生物化学，体内诱变和显微镜来表征RIM-BP 2相互作用物及其功能意义。我们将研究替代性突触特异性支架形成的假定机制，例如RIM-BP 2及其相互作用伙伴的同种型表达的变化，或蛋白质水平的变化。拟议中的实验有望揭示有助于活跃区功能结构的支架原理，并开辟对突触编码多样性背后的生物学的新理解。