Organization of the synaptic active zone scaffold by Bruchpilot and RIM-binding protein (A06)

Bruchpilot 和 RIM 结合蛋白 (A06) 组织突触活性区支架

• 批准号: 202199567
• 金额: --
• 依托单位: Arbeitsgruppe Strukturbiochemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/202199567?language=en
• 关键词: Organization; synaptic; active; zone; scaffold

Organization of the synaptic active zone scaffold by Bruchpilot and RIM-binding proteinNeurotransmission at chemical synapses depends on elaborate protein scaffolds that decorate active zones. In project A06, we aim at devising a pseudo-atomic model of the Bruchpilot/RIM-binding protein-based core of the active zone scaffold at Drosophila neuromuscular junctions. To this end, we will combine results from protein crystallography, biochemical structure probing, phylogenetic co-variation analysis, super-resolution light microscopy and cryo-electron tomography in an integrative structural modeling approach.

Bruchilot和RIM结合的蛋白质支架在化学突触上的组织依赖于装饰活动区的精心制作的蛋白质支架。在A06项目中，我们的目标是设计一个基于BruchPilot/RIM结合蛋白的果蝇神经肌肉接头活动区支架核心的伪原子模型。为此，我们将结合蛋白质结晶学、生化结构探测、系统发育协变分析、超分辨光学显微镜和冷冻电子断层扫描的结果，以一种综合的结构建模方法。