MRI: Acquisition of Nikon A1R-MP Spectral Confocal/Multiphoton Microscope with Widefield HyperSpectral Imaging System for the Jordan Hall Imaging Facility

这笔资金将使克莱姆森大学获得一个光谱共焦/多光子显微镜配备了宽视场光谱成像系统。新系统将被安置在约旦霍尔成像设施（JHIF），这是先进的光学显微镜核心成像设施在克莱姆森大学。JHIF的主要目标是获得和维护先进的光成像设备进行前沿研究所需的，从而提供theClemson研究社区获得必要的工具，与其他顶级研究机构竞争。作为一个核心设施，JHIF致力于培养从本科生和研究生到博士后研究人员的各级研究人员。为了与MRINSF的愿景保持一致，这种共享设备将提高克莱姆森的研究质量并扩大研究范围。克莱姆森的研究人员将利用多光子的能力来研究活细胞和整个生物体，如鸡胚、斑马鱼和秀丽隐杆线虫，因为这种技术提供了高分辨率的图像采集，比传统的共聚焦显微镜具有更低的光毒性和更深的分辨率。该仪器的宽视场高光谱功能将使目前从事纳米颗粒研究的克莱姆森研究人员受益，使他们不仅可以在真实的时间内可视化细胞对纳米颗粒的吸收，而且还可以识别这些纳米颗粒的化学特征。这些能力的增加将显著影响生物科学、生物工程等领域的研究质量。克莱姆森约旦霍尔成像设施致力于培养先进光学显微镜技术的本科生和研究生研究人员。此外，JHIF经理通过SCLife暑期课程向高中教师介绍先进的光学显微镜，让他们有机会使用这些技术。为了吸引和帮助科学界的弱势群体，JHIF提供了一个小型项目，在仪器上进行长达10小时的光束时间。这种显微镜将为克莱姆森研究人员带来更大的研究范围，这可能会给社会带来巨大的好处，包括保护环境、食品安全、新的药物输送系统以及免受生物恐怖主义威胁。

This funding will enable Clemson University to acquire a spectral confocal /multiphotonmicroscope equipped with a widefield spectral imaging system. The new system will be housedin the Jordan Hall Imaging Facility (JHIF), which is the advanced light microscopy core imagingfacility at Clemson University. The primary goal of the JHIF is to acquire and maintain advancedlight imaging equipment necessary for conducting leading edge research, thus, providing theClemson research community access to the tools necessary to compete with other top researchinstitutions. As a core facility, the JHIF is dedicated to the training of investigators at all levelsfrom undergraduate and graduate students to postdoctoral researchers. In keeping with the MRINSF vision, this shared equipment would improve the quality and expand the scope of researchat Clemson. The multiphoton capabilities will be utilized by Clemson researchers who work withlive cells and whole organisms, such as chick embryos, zebrafish, and Caenorhabditis elegans,as this technique provides high resolution image acquisition with less phototoxity and deeperresolution than conventional confocal microscopy. The widefield hyperspectral capabilities ofthis instrument will benefit Clemson researchers currently engaged in nanoparticle studies,allowing them to not only visualize uptake of nanoparticles by cells in real time, but to alsoidentify chemical characteristics of these nanoparticles. The addition of these capabilities willsignificantly influence the quality of research in the fields of biological science, bioengineering,The Clemson Jordan Hall Imaging Facility is dedicated to the training of undergraduate andgraduate researchers in advanced light microscopy techniques. In addition, the JHIF managerintroduces high school teachers to advanced light microscopy through SCLife summer courses,giving them an opportunity to work with these techniques. To attract and assist underrepresentedgroups in the sciences, the JHIF offers a minigrant program for up to 10 hours of beam time onthe instruments. The increased research scope that this microscope will bring to Clemsonresearchers could result in enormous benefits to society, including protecting the environment,food safety, new drug delivery systems, and safety from bioterrorism threats.