MRI: Acquisition of Nikon A1R-MP Spectral Confocal/Multiphoton Microscope with Widefield HyperSpectral Imaging System for the Jordan Hall Imaging Facility

这笔资金将使克莱姆森大学能够获得配备宽视场光谱成像系统的光谱共焦/多光子显微镜。新系统将安装在乔丹霍尔成像设备(JHIF)中，这是克莱姆森大学先进的光学显微镜核心成像设备。JHIF的主要目标是获取和维护开展前沿研究所需的先进光学成像设备，从而为克莱姆森研究界提供与其他顶尖研究机构竞争所需的工具。作为一个核心设施，JHIF致力于培训各级研究人员--从本科生和研究生到博士后研究人员。为了与MRINSF的愿景保持一致，这种共享设备将提高克莱姆森的质量并扩大研究范围。克莱姆森研究人员将利用多光子能力处理活细胞和整个生物体，如鸡胚胎、斑马鱼和秀丽线虫，因为这项技术提供了比传统共聚焦显微镜更低的光毒性和更高分辨率的图像采集。该仪器的广域高光谱能力将使目前从事纳米颗粒研究的克莱姆森研究人员受益，使他们不仅能够实时可视化细胞对纳米颗粒的摄取，还可以识别这些纳米颗粒的化学特征。这些能力的增加将显著影响生物科学、生物工程领域的研究质量，克莱姆森·乔丹霍尔成像设施致力于培训本科生和研究生研究人员先进的光学显微镜技术。此外，JHIF经理通过SCLife暑期课程向高中教师介绍高级光学显微镜，让他们有机会使用这些技术。为了吸引和帮助科学界中代表性不足的群体，JHIF提供了一项微型移民计划，最多10个小时的束流时间在仪器上。这种显微镜将为克莱姆森研究人员带来更大的研究范围，可能会给社会带来巨大的好处，包括保护环境、食品安全、新的药物输送系统以及免受生物恐怖主义威胁的安全。

This funding will enable Clemson University to acquire a spectral confocal /multiphotonmicroscope equipped with a widefield spectral imaging system. The new system will be housedin the Jordan Hall Imaging Facility (JHIF), which is the advanced light microscopy core imagingfacility at Clemson University. The primary goal of the JHIF is to acquire and maintain advancedlight imaging equipment necessary for conducting leading edge research, thus, providing theClemson research community access to the tools necessary to compete with other top researchinstitutions. As a core facility, the JHIF is dedicated to the training of investigators at all levelsfrom undergraduate and graduate students to postdoctoral researchers. In keeping with the MRINSF vision, this shared equipment would improve the quality and expand the scope of researchat Clemson. The multiphoton capabilities will be utilized by Clemson researchers who work withlive cells and whole organisms, such as chick embryos, zebrafish, and Caenorhabditis elegans,as this technique provides high resolution image acquisition with less phototoxity and deeperresolution than conventional confocal microscopy. The widefield hyperspectral capabilities ofthis instrument will benefit Clemson researchers currently engaged in nanoparticle studies,allowing them to not only visualize uptake of nanoparticles by cells in real time, but to alsoidentify chemical characteristics of these nanoparticles. The addition of these capabilities willsignificantly influence the quality of research in the fields of biological science, bioengineering,The Clemson Jordan Hall Imaging Facility is dedicated to the training of undergraduate andgraduate researchers in advanced light microscopy techniques. In addition, the JHIF managerintroduces high school teachers to advanced light microscopy through SCLife summer courses,giving them an opportunity to work with these techniques. To attract and assist underrepresentedgroups in the sciences, the JHIF offers a minigrant program for up to 10 hours of beam time onthe instruments. The increased research scope that this microscope will bring to Clemsonresearchers could result in enormous benefits to society, including protecting the environment,food safety, new drug delivery systems, and safety from bioterrorism threats.