Gametogenesis-related small non-coding RNAs and Argonaute proteins in Arabidopsis (B05)

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Small RNAs and Argonautes are central to gene regulation but analysis of these ribonucleoprotein (RNP) complexes in the flowering plant egg cell remains challenging because it is deeply buried inside the ovary. Project B5 aims to uncover small RNA profiles and RNP complexes of Arabidopsis egg cells and to investigate whether and how miRNAs in the egg cell impact cell specification, fertilization or embryogenesis. Furthermore, project B5 aims to elucidate the molecular mechanism(s) of cell-to-cell small RNA transfer in the microenvironment of the female gametophyte for intercellular communication.