RUI: Cell Wall Associated Protein Kinases As Pectin Receptors

RUI：细胞壁相关蛋白激酶作为果胶受体

• 批准号: 1146245
• 负责人: Bruce Kohorn
• 金额: $ 40万
• 依托单位: Bowdoin College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146245&HistoricalAwards=false
• 关键词: RUI; Cell; Wall; Associated; Protein

All organisms are made up of collections of cells, or bags of material that each carry out essential processes for life. Each cell has a program that can be influenced by the environment to enlarge, divide, and form specific shapes. The ultimate shape, size and function of a plant is therefore determined by the nature of each individual cell. Plant cells are surrounded by structurally strong but plastic material called the cell wall, that includes the carbohydrates cellulose and pectin. The cell wall has a direct influence on the size and shape that each cell reaches as it acts as a cage around each cell, and hence modification of the cell wall has a direct affect on cell shape and size. The cell surface contains a protein molecule, called a wall-associated kinase (WAK), that binds to the cell wall pectin and senses the structure and relays this to the inside of the cell to cause changes in growth and responses to the environment. Indeed, pathogens and wounding directly affect pectins, and WAKs aid in the sensing of this damage. WAKs are also required for normal plant growth. This project will concentrate on the mechanism by which WAKs bind and sense pectin changes so as to play a dual role differentiating growth from the stress response. Genetic and biochemical analysis using the model plant Arabidopsis will generate paradigms likely applicable to all types of flowering plants. Undergraduate students involved in the work will be provided with an integrative approach to science, and will be prepared for the challenges of graduate and professional schools and the private sector upon graduation.

所有生物体都是由细胞集合或材料袋组成的，每个细胞或材料袋都执行生命的基本过程。 每个细胞都有一个程序，可以受到环境的影响，扩大，分裂和形成特定的形状。因此，植物的最终形状、大小和功能是由每个细胞的性质决定的。植物细胞被称为细胞壁的结构坚固但具有可塑性的材料所包围，其中包括碳水化合物纤维素和果胶。细胞壁对每个细胞达到的大小和形状具有直接影响，因为它充当每个细胞周围的笼子，因此细胞壁的修饰对细胞形状和大小具有直接影响。 细胞表面含有一种蛋白质分子，称为壁相关激酶（WAK），它与细胞壁果胶结合并感知结构，并将其传递到细胞内部，以引起生长和对环境反应的变化。 事实上，病原体和创伤直接影响果胶，WAK有助于感知这种损伤。 WAK也是植物正常生长所必需的。本项目将集中于WAKs结合和感知果胶变化的机制，从而发挥区分生长和应激反应的双重作用。使用模式植物拟南芥的遗传和生化分析将产生可能适用于所有类型的开花植物的范例。参与这项工作的本科生将获得科学的综合方法，并将为毕业后研究生和专业学校以及私营部门的挑战做好准备。