Cell Wall Associated, Receptor Protein Kinases

细胞壁相关受体蛋白激酶

• 批准号: 9728045
• 负责人: Bruce Kohorn
• 金额: $ 37万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-15 至 2001-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728045&HistoricalAwards=false
• 关键词: Cell; Wall; Associated; Receptor; Protein

MCB9728045 Lay Abstract The interactions between the extracellular and intracellular compartments of cells play important roles in controlling growth and development. These interactions are essential for such basic cell functions as establishment of cell polarity and regulation of cell division, cell differentiation, cell migration and cell death. The extracellular compartment contains molecular components arranged in a complex architecture termed the extracellular matrix (ECM). It is well established that in animal cells a basic way in which the ECM can regulate cell processes is through its interaction with cell surface proteins (receptors) that bind to specific adhesive components of the ECM. By contrast, in plant cells, despite their overwhelming importance on earth, little is known about the communication between the ECM (the plant cell wall) and the cell interior. This proposal concerns a family of four cell surface receptors called Waks (short for wall-associated kinases), discovered in the cells of the flowering plant, Arabidopsis, which appear to be adhesion proteins that transmit signals between the extracellular and intracellular compartments. Using genetics, molecular biology, biochemistry, and microscopy, we will determine the tissue location of these receptors and the molecular mechanism by which they mediate communication between the inside and outside of the plant cell. We propose that the Waks generate a signal inside the cell by their ability to interact with and to modify (phosphorylate) specific target proteins which then initiate a cascade of molecular events within the cytoplasm. This proposal describes experiments to dissect these interactions and events. The analysis is likely to elucidate general principles of how eukaryotic cells interact with their immediate environment and perceive changes in the architecture of their ECM.

MCB 9728045 通俗摘要 细胞的细胞外区室和细胞内区室之间的相互作用在控制生长和发育中起重要作用。 这些相互作用对于细胞基本功能如细胞极性的建立和细胞分裂、细胞分化、细胞迁移和细胞死亡的调节是必不可少的。 细胞外区室含有排列在称为细胞外基质（ECM）的复杂结构中的分子组分。 已经确定的是，在动物细胞中，ECM可以调节细胞过程的基本方式是通过其与细胞表面蛋白（受体）的相互作用，所述细胞表面蛋白与ECM的特定粘附组分结合。 相比之下，在植物细胞中，尽管它们在地球上具有压倒性的重要性，但对ECM（植物细胞壁）和细胞内部之间的通信知之甚少。 该提议涉及一个称为Waks（壁相关激酶的缩写）的四种细胞表面受体家族，该家族在开花植物拟南芥的细胞中发现，似乎是在细胞外和细胞内隔室之间传递信号的粘附蛋白。 利用遗传学，分子生物学，生物化学和显微镜，我们将确定这些受体的组织位置和它们介导植物细胞内外之间通信的分子机制。 我们提出，Waks通过与特定靶蛋白相互作用并修饰（磷酸化）特定靶蛋白的能力在细胞内产生信号，然后在细胞质内启动分子事件级联。 该提案描述了剖析这些相互作用和事件的实验。 该分析可能阐明真核细胞如何与其直接环境相互作用并感知其ECM结构变化的一般原理。