Quantification of Organic Nitrogen use in FACE and Culture Experiments

FACE 和培养实验中有机氮使用的量化

• 批准号: 1146328
• 负责人: Erik Hobbie
• 金额: $ 32.32万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146328&HistoricalAwards=false
• 关键词: Quantification; Organic; Nitrogen; use; FACE

Much of plant nitrogen supply, particularly for trees, is supplied through symbiotic (mycorrhizal) fungi that obtain sugars from their host trees and in return transfer nitrogen. However, methodological difficulties have limited quantification of the role that mycorrhizal fungi play in meeting plant nitrogen demands. In this project, ratios of carbon and nitrogen isotopes from two field experiments at Duke University and at Rhinelander, Wisconsin will be used to assess organic nitrogen uptake by different mycorrhizal fungi and plants. This information will be combined with laboratory studies on mycorrhizal plants to provide the first realistic and quantitative estimates of organic nitrogen use. New methodologies being developed for this project will determine whether patterns of organic nitrogen use differ across ecosystems and across species of mycorrhizal fungi.This work will develop and perfect new methods to quantify organic nitrogen use by plants and mycorrhizal fungi which will transform how researchers think about nitrogen cycling in the soil and its connections to soil carbon. This has implications for forest management. Educational work will involve collaboration with several local high school teachers to expand their use of outdoor classrooms and use of fungi as a tool to teach biology. Outreach to the public will also include educating Appalachian Mountain Club (AMC) naturalists on fungal roles in forests as well as leading AMC nature walks. The project will involve training for a Ph.D. student and several undergraduates, including recruitment of students from Elizabeth City State University, a historically black institution with strong academic ties to UNH.

植物的大部分氮供应，特别是树木，是通过共生（菌根）真菌提供的，这些真菌从它们的宿主树木中获取糖，并反过来转移氮。然而，方法上的困难限制了菌根真菌在满足植物氮需求方面的作用的量化。在这个项目中，来自杜克大学和威斯康辛州莱茵兰德的两个实地实验的碳和氮同位素比率将被用来评估不同菌根真菌和植物对有机氮的吸收。这些信息将与对菌根植物的实验室研究相结合，提供有机氮利用的第一个现实的定量估计。正在为这个项目开发的新方法将确定有机氮的使用模式是否在不同的生态系统和不同种类的菌根真菌中有所不同。这项工作将开发和完善新的方法来量化植物和菌根真菌对有机氮的利用，这将改变研究人员对土壤中氮循环及其与土壤碳的联系的看法。这对森林管理有影响。教育工作将包括与几位当地高中教师合作，扩大户外教室的使用，并将真菌作为一种工具来教授生物学。与公众的接触还包括教育阿巴拉契亚山俱乐部（AMC）的自然学家了解真菌在森林中的作用，以及带领AMC自然漫步。该项目将包括对一名博士生和几名本科生的培训，包括从伊丽莎白市立大学（Elizabeth City State University）招募学生，这是一所历史悠久的黑人大学，与新罕布什尔大学有着密切的学术联系。