Molecular Scale Study of Peptide Interaction with Metal Oxide Nanoparticles

肽与金属氧化物纳米颗粒相互作用的分子尺度研究

• 批准号: 1152604
• 负责人: Joel Pedersen
• 金额: $ 43.3万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1152604&HistoricalAwards=false
• 关键词: Molecular; Scale; Study; Peptide; Interaction

The Environmental Chemical Sciences (ECS) program of the Division of Chemistry will support the research program of Profs. Joel Pedersen and Robert Hamers of University of Wisconsin, Madison. Profs. Pedersen and Hamers and their students will investigate the interaction of well-defined peptides with metal oxide nanoparticles as a first step toward a deeper understanding of how nanoparticles interact with proteins. Titanium dioxide and aluminum oxide will be used as model systems for investigation. The specific aims of the study are: (i) employ discovery tools (phage-display methods) to identify binding motifs in short peptide sequences (heptapeptides) that lead to high affinity for nanoparticles of environmental relevance; (ii) investigate the thermodynamics and kinetics of peptide binding to TiO2 and Al2O3 nanoparticles, using both binding sequences identified via phage-display methods and simpler combinations of amino acids in tetrapeptides to elucidate binding rules; and (iii) identify the peptide functional groups that interact with the nanoparticles and understand the nature of peptide-surface bonds by combining experimental with computational methods. The outcome of this research will be a fundamental understanding of the molecular level interactions that control the interactions of peptides with metal oxide nanoparticles. Knowledge of how factors such as peptide sequence, nanoparticle composition and structure, and solution composition affect peptide-nanoparticle interactions will provide insights into the physical and chemical processes that ultimately control the environmental safety and health impacts of nanomaterials. The project will provide outstanding educational opportunities for graduate and undergraduate students desiring to understand the interface between nanomaterials and biological systems.

化学系的环境化学科学(ECS)计划将支持教授的研究计划。麦迪逊威斯康星大学的乔尔·彼得森和罗伯特·哈默斯。教授们。Pedersen和Hamers以及他们的学生将研究定义明确的多肽与金属氧化物纳米颗粒的相互作用，作为深入了解纳米颗粒与蛋白质相互作用的第一步。二氧化钛和氧化铝将作为研究的模型体系。这项研究的具体目的是：(I)利用发现工具(噬菌体展示法)确定短肽序列(七肽)中的结合基序，从而导致与环境相关的纳米颗粒的高亲和力；(Ii)利用噬菌体展示法鉴定的结合序列和四肽中较简单的氨基酸组合来研究多肽与纳米颗粒结合的热力学和动力学，以阐明结合规则；以及(Iii)通过实验和计算相结合的方法，鉴定与纳米颗粒相互作用的多肽官能团，了解多肽-表面键的性质。这项研究的结果将是对控制多肽与金属氧化物纳米颗粒相互作用的分子水平相互作用的基本了解。了解多肽序列、纳米颗粒组成和结构以及溶液组成等因素如何影响多肽-纳米颗粒相互作用，将有助于深入了解最终控制纳米材料环境安全和健康影响的物理和化学过程。该项目将为希望了解纳米材料和生物系统之间的接口的研究生和本科生提供绝佳的教育机会。