Quantification of the Biotransport Phenomena in Low-Cryoprotectant (CPA) Vitrification of Living Cells

活细胞低冷冻保护剂 (CPA) 玻璃化冷冻中生物转运现象的定量

• 批准号: 1154965
• 负责人: Xiaoming He
• 金额: $ 27.39万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1154965&HistoricalAwards=false
• 关键词: Quantification; Biotransport; Phenomena; Low; Cryoprotectant

1033426HeContemporary methods for cell cryopreservation often produce a low yield of viable cells due to the harmful effects of ice formation or use of a high concentration of cryoprotectant (CPA). This research seeks to achieve ice-free cryopreservation (vitrification) of cells using a low, nontoxic concentration of CPA (low-CPA). A quantitative understanding of the thermally driven, non-equilibrium phase transition of water in living cells into either a crystalline or a glassy solid, and its correlation with cell survival during low-CPA vitrification has not been achieved to date. Therefore, the objective of this project is to quantify the relevant fundamental phenomena by model development coupled with experimental verification.Intellectual Merit: A coupled thermal and phase transition model will be developed which will facilitate quantitative understanding of the pertinent biotransport processes during low-CPA vitrification. Optimal low-CPA vitrification protocols for cryopreserving various living cells will be determined. Broader Impacts: The proposed research will assist the advancement of emerging cell-based medicine of which the eventual success relies upon the capability to preserve living cells for distribution to end users. Moreover, the proposed research will be integrated into education through (a) curriculum development, (b) training of graduate, undergraduate, and high school students, and (c) dissemination of the research findings including by a specialized web page.

1033426He 由于冰形成或使用高浓度冷冻保护剂 (CPA) 的有害影响，现代细胞冷冻保存方法通常会产生低产量的活细胞。本研究旨在使用低、无毒浓度的 CPA (low-CPA) 实现细胞的无冰冷冻保存（玻璃化）。迄今为止，尚未对活细胞中的水受热驱动、非平衡相转变为结晶或玻璃状固体的定量理解，及其与低 CPA 玻璃化过程中细胞存活的相关性。因此，该项目的目标是通过模型开发和实验验证来量化相关的基本现象。智力优点：将开发耦合热和相变模型，这将有助于定量理解低 CPA 玻璃化过程中的相关生物转运过程。将确定冷冻保存各种活细胞的最佳低 CPA 玻璃化冷冻方案。更广泛的影响：拟议的研究将有助于新兴细胞医学的进步，其最终成功取决于保存活细胞以分发给最终用户的能力。此外，拟议的研究将通过以下方式融入教育：（a）课程开发，（b）研究生、本科生和高中生的培训，以及（c）通过专门网页等方式传播研究成果。