Quantification of the Biotransport Phenomena in Low-Cryoprotectant (CPA) Vitrification of Living Cells
活细胞低冷冻保护剂 (CPA) 玻璃化冷冻中生物转运现象的定量
基本信息
- 批准号:1154965
- 负责人:
- 金额:$ 27.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-07-01 至 2015-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1033426HeContemporary methods for cell cryopreservation often produce a low yield of viable cells due to the harmful effects of ice formation or use of a high concentration of cryoprotectant (CPA). This research seeks to achieve ice-free cryopreservation (vitrification) of cells using a low, nontoxic concentration of CPA (low-CPA). A quantitative understanding of the thermally driven, non-equilibrium phase transition of water in living cells into either a crystalline or a glassy solid, and its correlation with cell survival during low-CPA vitrification has not been achieved to date. Therefore, the objective of this project is to quantify the relevant fundamental phenomena by model development coupled with experimental verification.Intellectual Merit: A coupled thermal and phase transition model will be developed which will facilitate quantitative understanding of the pertinent biotransport processes during low-CPA vitrification. Optimal low-CPA vitrification protocols for cryopreserving various living cells will be determined. Broader Impacts: The proposed research will assist the advancement of emerging cell-based medicine of which the eventual success relies upon the capability to preserve living cells for distribution to end users. Moreover, the proposed research will be integrated into education through (a) curriculum development, (b) training of graduate, undergraduate, and high school students, and (c) dissemination of the research findings including by a specialized web page.
由于冰形成的有害影响或使用高浓度的冷冻保护剂(CPA),用于细胞冷冻保存的当代方法通常产生低产量的活细胞。本研究旨在使用低无毒浓度的CPA(低CPA)实现细胞的无冰冷冻保存(玻璃化)。到目前为止,尚未实现对活细胞中水的热驱动非平衡相变为结晶或玻璃状固体及其与低CPA玻璃化期间细胞存活的相关性的定量理解。因此,本项目的目标是量化相关的基本现象,模型开发加上实验verified.Intellectual优点:一个耦合的热和相变模型将开发,这将有助于定量了解相关的生物运输过程中低CPA玻璃化。将确定用于冷冻保存各种活细胞的最佳低CPA玻璃化方案。更广泛的影响:拟议的研究将有助于新兴的基于细胞的医学的发展,其最终的成功取决于保存活细胞以分发给最终用户的能力。此外,拟议的研究将通过以下方式纳入教育:(a)课程编制;(B)对研究生、大学生和高中生进行培训;(c)传播研究成果,包括通过专门网页。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Xiaoming He其他文献
Chieh-qua Biotechnology: P rogress and P rospect s
捷夸生物科技:进展与展望
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
Qinghua Chen;Xiaoming He;Dasen Xie;Qingwu Peng - 通讯作者:
Qingwu Peng
Additive Manufacturing of Spent Fuel Storage Rack Model by Selective Laser Melting
选择性激光熔化增材制造乏燃料储存架模型
- DOI:
10.1115/nuclrf2018-7409 - 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Xiaoming He;Ziqiang Zhu;Changlei Shao;Ran Huang - 通讯作者:
Ran Huang
CEO leadership, strategic decision comprehensiveness and firm performance: The moderating role of TMT collectivistic orientation
CEO领导力、战略决策全面性与公司绩效:TMT集体主义导向的调节作用
- DOI:
- 发表时间:
- 期刊:
- 影响因子:2.9
- 作者:
Yaqun Yi;Yu Chen;Xiaoming He - 通讯作者:
Xiaoming He
High-performance 2.5 V flexible aqueous asymmetric supercapacitors based on K+/Na+-inserted MnO2 nanosheets
基于插入 K/Na 的 MnO2 纳米片的高性能 2.5 V 柔性水性不对称超级电容器
- DOI:
10.1016/j.electacta.2019.01.087 - 发表时间:
2019-03 - 期刊:
- 影响因子:6.6
- 作者:
Yangyang Liu;Lixia Guo;Xue Teng;Jianying Wang;Tianzi Hao;Xiaoming He;Zuofeng Chen - 通讯作者:
Zuofeng Chen
miRNA-145 and IGF-1R in hydrosalpinx-induced infertility
miRNA-145 和 IGF-1R 在输卵管积水引起的不孕症中的作用
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Zhi;Zhi;Kuiming Jiang;Shiwei Mei;Xiaoming He;Lianguo Zhang - 通讯作者:
Lianguo Zhang
Xiaoming He的其他文献
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{{ truncateString('Xiaoming He', 18)}}的其他基金
Collaborative Research: Models, algorithms, simulations and applications for dendritic solidifications of two-phase multi-component alloys in the mushy zone
合作研究:糊状区两相多组分合金枝晶凝固的模型、算法、模拟和应用
- 批准号:
2309733 - 财政年份:2023
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
Collaborative Research: Laboratory Data Enabled Phase Field Modeling and Data Assimilation for Coupled Two-Phase Fluid Flow and Porous Media Flow
合作研究:耦合两相流体流和多孔介质流的实验室数据支持相场建模和数据同化
- 批准号:
2152609 - 财政年份:2022
- 资助金额:
$ 27.39万 - 项目类别:
Continuing Grant
Midwest Numerical Analysis Day 2020
2020 年中西部数值分析日
- 批准号:
2023264 - 财政年份:2020
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
Collaborative Research: Models, Algorithms, and Simulations for Two-Phase Ferrofluid Flows in Contact with a Solid Surface
合作研究:与固体表面接触的两相铁磁流体流动的模型、算法和模拟
- 批准号:
1818642 - 财政年份:2018
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
Collaborative Research: Data-enabled Modeling, Numerical Method, and Data Assimilation for Coupling Dual Porosity Flow with Free Flow
协作研究:双孔隙流动与自由流动耦合的数据建模、数值方法和数据同化
- 批准号:
1722647 - 财政年份:2017
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
SusCHEM: Fundamental Studies on Lyopreservation of Adult Stem Cells
SusCHEM:成体干细胞冷冻保存的基础研究
- 批准号:
1831019 - 财政年份:2017
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
SusCHEM: Fundamental Studies on Lyopreservation of Adult Stem Cells
SusCHEM:成体干细胞冷冻保存的基础研究
- 批准号:
1605425 - 财政年份:2016
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
Non-Iterative Multi-Physics Domain Decomposition Method for the Navier-Stokes-Darcy Model
纳维-斯托克斯-达西模型的非迭代多物理域分解方法
- 批准号:
1418624 - 财政年份:2014
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
Quantification of the Biotransport Phenomena in Low-Cryoprotectant (CPA) Vitrification of Living Cells
活细胞低冷冻保护剂 (CPA) 玻璃化冷冻中生物转运现象的定量
- 批准号:
1033426 - 财政年份:2010
- 资助金额:
$ 27.39万 - 项目类别:
Standard Grant
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