Quantification of the Biotransport Phenomena in Low-Cryoprotectant (CPA) Vitrification of Living Cells

由于冰形成的有害影响或使用高浓度的冷冻保护剂（CPA），用于细胞冷冻保存的当代方法通常产生低产量的活细胞。本研究旨在使用低无毒浓度的CPA（低CPA）实现细胞的无冰冷冻保存（玻璃化）。到目前为止，尚未实现对活细胞中水的热驱动非平衡相变为结晶或玻璃状固体及其与低CPA玻璃化期间细胞存活的相关性的定量理解。因此，本项目的目标是量化相关的基本现象，模型开发加上实验verified.Intellectual优点：一个耦合的热和相变模型将开发，这将有助于定量了解相关的生物运输过程中低CPA玻璃化。将确定用于冷冻保存各种活细胞的最佳低CPA玻璃化方案。更广泛的影响：拟议的研究将有助于新兴的基于细胞的医学的发展，其最终的成功取决于保存活细胞以分发给最终用户的能力。此外，拟议的研究将通过以下方式纳入教育：（a）课程编制;（B）对研究生、大学生和高中生进行培训;（c）传播研究成果，包括通过专门网页。

1033426HeContemporary methods for cell cryopreservation often produce a low yield of viable cells due to the harmful effects of ice formation or use of a high concentration of cryoprotectant (CPA). This research seeks to achieve ice-free cryopreservation (vitrification) of cells using a low, nontoxic concentration of CPA (low-CPA). A quantitative understanding of the thermally driven, non-equilibrium phase transition of water in living cells into either a crystalline or a glassy solid, and its correlation with cell survival during low-CPA vitrification has not been achieved to date. Therefore, the objective of this project is to quantify the relevant fundamental phenomena by model development coupled with experimental verification.Intellectual Merit: A coupled thermal and phase transition model will be developed which will facilitate quantitative understanding of the pertinent biotransport processes during low-CPA vitrification. Optimal low-CPA vitrification protocols for cryopreserving various living cells will be determined. Broader Impacts: The proposed research will assist the advancement of emerging cell-based medicine of which the eventual success relies upon the capability to preserve living cells for distribution to end users. Moreover, the proposed research will be integrated into education through (a) curriculum development, (b) training of graduate, undergraduate, and high school students, and (c) dissemination of the research findings including by a specialized web page.