Collaborative Research: Cyanate availability and utilization by marine microbial assemblages

合作研究：海洋微生物群落的氰酸盐可用性和利用

• 批准号: 1155666
• 负责人: Margaret Mulholland
• 金额: $ 15万
• 依托单位: Old Dominion University Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1155666&HistoricalAwards=false
• 关键词: Collaborative; Research; Cyanate; availability; utilization

Researchers from the Marine Biological Laboratory at Woods Hole and from Old Dominion University will continue to develop a HPLC method for the measurement of cyante in seawater and will use this to demonstrate that cynate is present at measureable and environmentally relevant concentrations in natural water samples. It is hypothsized that cyanate could be an important source of nitrogen (N) to microbes and potentially also a source of carbon, for both phototrophic and heterotrophic organisms. Once the method is developed and tested the investigators will perform experiments in the laboratory primarily to examine the importance of cyante as a N source to microbes, and to demonstrate that cyanate itself rather than a breakdown product is assimialted and incorporated into cellular material. The studies will also involve coastal sampling focusing on Synechococcus and the identification of the organisms that assimilate cyanate, and examine the expression of the related genes. These preliminary studies will provide the foundation for more detailed future studies. This study will contribute to an improved understanding of the importance of small molecular weight organic N compounds as a source of N for organism growth and will build on other studies examining the importance of organic N compunds in the marine environment. The project will be part of the studies of a graduate student and fund other researchers and the investigators will incorporate their findings into current outreach efforts at all levels.

来自伍兹霍尔海洋生物实验室和奥多明尼恩大学的研究人员将继续开发一种用于测量海水中氰酸盐的 HPLC 方法，并将利用该方法来证明氰酸盐在天然水样中以可测量且与环境相关的浓度存在。据推测，氰酸盐可能是微生物的重要氮源（N），也可能是光养生物和异养生物的碳源。一旦开发并测试了该方法，研究人员将在实验室中进行实验，主要是为了检查氰酸盐作为微生物氮源的重要性，并证明氰酸盐本身而不是分解产物被同化并融入细胞材料中。这些研究还将涉及沿海取样，重点关注聚球藻和同化氰酸盐的生物体的鉴定，并检查相关基因的表达。这些初步研究将为未来更详细的研究奠定基础。这项研究将有助于更好地了解小分子量有机氮化合物作为生物体生长氮源的重要性，并将建立在其他研究有机氮化合物在海洋环境中的重要性的基础上。该项目将成为研究生研究的一部分，并资助其他研究人员，研究人员将把他们的发现纳入当前各级的推广工作中。