SAVI: Yeast Chromosome (Sc2.0) Synthesis and Analysis

SAVI：酵母染色体 (Sc2.0) 合成与分析

• 批准号: 1158201
• 负责人: Jef Boeke
• 金额: $ 50万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-15 至 2014-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1158201&HistoricalAwards=false
• 关键词: SAVI; Yeast; Chromosome; Sc2.0; Synthesis

The objective of this SAVI is to create a "Virtual Institute" that will support the synthesis of an entire eukaryotic genome. Writing a complete eukaryotic genome remains daunting and beyond the proven DNA synthesis capabilities of any individual lab. In analogy to the Human Genome Project, these first efforts will require the coordinated activities of many individual centers. This proposal will put in place the research coordination infrastructure to enable the complete synthesis of the Saccharomyces cerevisiae genome within 5 years at sites spanning the USA, Asia, and Europe, at institutions ranging from four-year liberal arts colleges to major research institutions, thereby capturing the imagination of undergraduate students, fellows, staff and faculty members worldwide.The specific objectives of this SAVI initiative are to solidify current collaborations between researchers at Johns Hopkins University, Loyola University of Maryland, and international partners in China, India and Europe. The strategic outsourcing of the synthesis of individual chromosomes guarantees that the entire yeast genome is completed within a five-year period with leveraged funds and resources from the US and international participating partners and funding agencies. Broader Impacts - The broader impacts of this SAVI project are the first-ever ability to ask and answer deep questions about fundamental properties of chromosomes, genome organization, gene content, the function of RNA splicing, the distinction between prokaryotes and eukaryotes, and numerous other aspects of genomes and evolution. The "synthetic yeast" will play an important role in practical applications, ranging from a test-bed for focused biological problems to a chassis for future synthetic biology projects. Notably, yeast is the preeminent organism used for industrial fermentations, with a wide variety of practical uses, including ethanol production from agricultural products and by-products. As the only effort of its kind outside industry, this project provides a unique and very important service in keeping the knowledge in the Synthetic Biology field public through open source availability of all reagents and protocols without intellectual property restrictions. This SAVI effort is unique also in training researchers in genome-scale synthetic Biology. An innovative "Build A Genome" course was developed and implemented at Johns Hopkins University, which has since been offered at Loyola Univ. of Maryland, and two universities in China. The SAVI imitative will also offer opportunities for multi-disciplinary training of students, postdocs, and researchers, and will encourage international exchange.This award is designated as a Science Across Virtual Institutes (SAVI) award and is being co-funded by NSF's Directorate for Biological Sciences, Directorate for Engineering, and Office of International Science and Engineering.

SAVI的目标是创建一个“虚拟研究所”，支持整个真核基因组的合成。编写一个完整的真核基因组仍然令人生畏，超出了任何单个实验室的DNA合成能力。与人类基因组计划类似，这些最初的努力将需要许多单独中心的协调活动。该提案将建立研究协调基础设施，使酿酒酵母基因组的完整合成在5年内在横跨美国，亚洲和欧洲的地点，在机构范围从四年制文理学院到主要研究机构，从而捕捉本科生，研究员，SAVI计划的具体目标是巩固约翰霍普金斯大学、马里兰州洛约拉大学的研究人员与中国、印度和欧洲的国际合作伙伴之间的合作。单个染色体合成的战略外包保证了整个酵母基因组在五年内完成，并利用来自美国和国际参与合作伙伴和资助机构的杠杆资金和资源。更广泛的影响-这个SAVI项目的更广泛的影响是有史以来第一次能够提出和回答关于染色体的基本属性，基因组组织，基因内容，RNA剪接的功能，原核生物和真核生物之间的区别以及基因组和进化的许多其他方面的深层次问题。“合成酵母”将在实际应用中发挥重要作用，从重点生物学问题的试验台到未来合成生物学项目的底盘。值得注意的是，酵母是用于工业发酵的杰出生物，具有广泛的实际用途，包括从农产品和副产品生产乙醇。作为行业外唯一的此类努力，该项目提供了一个独特而非常重要的服务，通过开源所有试剂和协议，在没有知识产权限制的情况下，保持合成生物学领域的知识公开。SAVI的努力在培养基因组规模合成生物学研究人员方面也是独一无二的。约翰霍普金斯大学开发并实施了一个创新的“构建基因组”课程，该课程已在马里兰州的洛约拉大学和中国的两所大学开设。SAVI模拟奖还将为学生、博士后和研究人员提供多学科培训的机会，并鼓励国际交流。该奖项被指定为跨虚拟研究所科学奖（SAVI），由NSF生物科学理事会、工程理事会和国际科学与工程办公室共同资助。