IRFP: Scanless Two-Photon Voltage Imaging of Live Neuron Activity With Holographic Wavefront Shaping

IRFP：利用全息波前整形对活体神经元活动进行无扫描双光子电压成像

• 批准号: 1159089
• 负责人: Amanda Foust
• 金额: $ 14.17万
• 依托单位: Foust Amanda J
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1159089&HistoricalAwards=false
• 关键词: IRFP; Scanless; Two; Photon; Voltage

The International Research Fellowship Program enables U.S. scientists and engineers to conduct nine to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twenty-four month research fellowship by Dr. Amanda J. Foust to work with Dr. Valentina Emiliani at Université Paris Descartes, Paris, France. Voltage imaging has the potential to revolutionize our understanding of how neuronal cells and circuits process and store information through a mixture of analog and digital electrical signaling in the living brain. However, low signal-to-noise ratios and high background have prevented spatial and temporal resolution adequate for tracking electrical activity on cellular and subcellular length scales in-vivo. Light sculpturing techniques such as Gradient Phase Contrast (GPC) and temporal focusing (TF) under development in Dr. Valentina Emiliani's laboratory can improve the spatial resolution of voltage imaging techniques by enabling targeted fluorescence excitation of specified cells and substructures. To this end, we are developing, adapting, and optimizing wavefront shaping technology for imaging the propagation of electrical activity in the axons and dendrites of neurons with voltage-sensitive dyes and fluorescent proteins. Development of wavefront shaping technology for spatially targeted voltage sensing will provide a powerful tool for investigating the electrical underpinnings of sensation, perception, cognition, and behavior. Moreover, these techniques can be used to determine what fundamental circuit alterations occur with neurological diseases and disorders such as epilepsy and multiple sclerosis, and inform development of more effective therapeutic strategies.

国际研究奖学金计划使美国科学家和工程师能够在国外进行9至24个月的研究。该计划的奖项提供了联合研究的机会，以及使用国外独特或互补的设施，专业知识和实验条件。该奖项将支持阿曼达J.福斯特博士在法国巴黎的巴黎笛卡尔大学与Valentina Emiliani博士合作的24个月研究奖学金。电压成像有可能彻底改变我们对神经元细胞和电路如何通过模拟和数字电信号在活体大脑中混合处理和存储信息的理解。然而，低信噪比和高背景阻止了空间和时间分辨率足以在体内跟踪细胞和亚细胞长度尺度上的电活动。 Valentina Emiliani博士实验室正在开发的光雕刻技术，如梯度相位对比（GPC）和时间聚焦（TF），可以通过实现特定细胞和子结构的靶向荧光激发来提高电压成像技术的空间分辨率。为此，我们正在开发，调整和优化波前整形技术，用于用电压敏感染料和荧光蛋白成像神经元轴突和树突中的电活动传播。波前整形技术的发展，空间目标电压传感将提供一个强大的工具，调查的感觉，知觉，认知和行为的电基础。 此外，这些技术可用于确定癫痫和多发性硬化等神经系统疾病和障碍发生的基本回路改变，并为更有效的治疗策略的开发提供信息。