RUI: Unlock the Potential of Three-Dimensional (3D) Cell Cultures as Sensing Elements in Label-free Impedance Biosensors

RUI：释放三维 (3D) 细胞培养物作为无标记阻抗生物传感器中传感元件的潜力

• 批准号: 1159871
• 负责人: Liju Yang
• 金额: $ 25.59万
• 依托单位: North Carolina Central University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1159871&HistoricalAwards=false
• 关键词: RUI; Unlock; Potential; Three; Dimensional

Yang1159871 This project is to develop 3D cell culture-based impedance biosensors to provide label?free, non-invasive and real-time analytical tools for cellular analysis and drug discovery applications. This is based on the fact that 3D cell cultures resemble more accurately the actual in vivo microenvironment than the conventional 2D cell culture, thus 3D cell culture-based biosensors are expected to provide more biologically relevant and predictive data for in vivo responses. The project will design a microcavity array-based microchip for constructing the impedance biosensor and testing two types of 3D cell cultures--spheroids and cells embedded in 3D matrixes as the sensing element. The research tasks include the following: (1) studying the feasibility of such 3D culture-based biosensors for sensing drug-induced cell signaling pathways; (2) comparing the dose response range and IC50 of the 2D and 3D cell?based biosensors to drugs with different action mechanisms; (3) testing three different prostate cancer cell lines with different adherence characteristics (adherent, poorly adherent, non-adherent) for constructing 3D cultures as sensing elements and comparing their responses to drugs in comparison with traditional 2D cell culture tests. The project will evaluate the capability of the proposed 3D culture-based biosensors in reflecting the difference between 3D and 2D cultures, and to determine the potential of such 3D culture-based impedance biosensors in providing more biologically relevant information. This project will particularly address the research and training needs for students from underrepresented groups at North Carolina Central University (NCCU). NCCU is one of the historic black college and universities (HBCU). The education activities include (1) integrating research into course development; (2) providing research opportunities for undergraduate students through lab modules, summer internships, and external training opportunities; (3) broadening students? research vision by inviting external speakers to the interdisciplinary seminar series; (4) working with two existing outreach programs, the BRITE Futures and the Summer venture program, to educate k-12 students by exposure to research. The goal is to broaden students? knowledge in the fast developing biosensor and biochip related analytical technologies, and make students aware that real-life contemporary biological problems can be addressed by engineered microdevices.

杨1159871该项目旨在开发基于3D细胞培养的阻抗生物传感器，为细胞分析和药物发现应用提供无标签、非侵入性和实时的分析工具。这是基于这样一个事实，即3D细胞培养比传统的2D细胞培养更准确地模拟体内的实际微环境，因此基于3D细胞培养的生物传感器有望为体内反应提供更多的生物学相关和预测数据。该项目将设计一种基于微腔阵列的微芯片，用于构建阻抗生物传感器，并测试两种类型的3D细胞培养--球体和嵌入3D基质中的细胞作为传感元件。研究任务包括：(1)研究这种基于3D培养的生物传感器用于传感药物诱导的细胞信号通路的可行性；(2)比较2D和3D细胞生物传感器对不同作用机制药物的剂量响应范围和IC50；(3)测试三种不同黏附特性(粘附性、粘附性和非粘附性)的前列腺癌细胞系，以构建3D培养作为传感元件，并与传统的2D细胞培养测试比较它们对药物的响应。该项目将评估拟议的基于3D培养的生物传感器在反映3D和2D培养之间的差异方面的能力，并确定这种基于3D培养的阻抗生物传感器在提供更多生物相关信息方面的潜力。该项目将特别针对北卡罗来纳中央大学(NCCU)代表不足群体的学生的研究和培训需求。NCCU是历史悠久的黑人学院和大学之一。教育活动包括(1)将研究融入课程开发；(2)通过实验模块、暑期实习和外部培训机会为本科生提供研究机会；(3)拓宽学生范围？通过邀请外部演讲者参加跨学科研讨会系列，展示研究视野；(4)与现有的两个外展计划--BRITE未来计划和夏季创业计划--合作，通过接触研究来教育K-12学生。我们的目标是拓宽学生范围？具有快速发展的生物传感器和生物芯片相关分析技术的知识，并使学生意识到现实生活中的当代生物学问题可以通过工程微型设备来解决。