Fluorescence-Enabled Electrochemical Detection and Imaging

华盛顿大学张博教授在化学系化学测量与成像项目的支持下，引入并利用基于荧光的分析方法来提高电化学检测的灵敏度。该方法的一个关键概念是使用荧光反应来报告感兴趣的常规电化学反应的动力学，例如多巴胺的氧化。因此，该方法可以显著提高电化学检测的灵敏度，实现新的能力。该项目将侧重于对双极微电极上的偶联反应进行更全面的了解，并优化荧光电化学检测以实现单分子灵敏度。这种新的分析策略可能对许多重要领域产生重大影响，如神经化学、环境分析和电催化。这项工作将为电化学、传感和荧光显微镜领域的研究生和本科生提供极好的培训机会。研究生和本科生，包括女学生和少数民族学生，将被招募到这个研究项目中。他们将积极参与许多培训机会，如使用最先进的分析仪器，设计新的电化学实验和分析结果，在小组会议和参加国家会议上发言，并在该领域的主要期刊上发表文章。

Professor Bo Zhang of the University of Washington is supported by the Chemical Measurement and Imaging Program in the Division of Chemistry to introduce and utilize a fluorescence-based analytical method to increase the sensitivity of electrochemical detection. A key concept of this method is to use a fluorogenic reaction to report the kinetics of a conventional electrochemical reaction of interest, e.g., oxidation of dopamine. As such, this method could significantly improve sensitivity in electrochemical detection and enable new capability. This project will focus on developing a more comprehensive understanding of coupled reactions on bipolar microelectrodes and optimizing fluorescence-enabled electrochemical detection to achieve single-molecule sensitivity. This new analytical strategy could have significant impact on many important fields, such as neurochemistry, environmental analysis, and electrocatalysis. This work will provide excellent training opportunities for graduate and undergraduate students in the areas of electrochemistry, sensing, and fluorescence microscopy. Graduate and undergraduate students, including female students and minority students, will be recruited into this research program. They will be actively involved in numerous training opportunities such as using state-of-the-art analytical instruments, designing new electrochemical experiments and analyzing results, presenting at group meetings and attending national conferences, and publishing in leading journals in this field.