Fluorescence-Enabled Electrochemical Microscopy

该奖项由化学系化学测量和成像项目资助，华盛顿大学的张波教授正在开发一种新的分析显微镜方法;即荧光电化学显微镜（FEEM），用于成像大型电化学阵列的瞬态动态氧化还原过程。这项研究的一个基本概念是将电信号转换为荧光，以便光学显微镜可以用于监测氧化还原物质，如多巴胺。 该项目将开发FEEM作为理解大脑中分子运输，神经元活动和神经元-神经元通信的工具。通过招募女性和代表性不足群体的成员参与这门科学来解决更广泛的影响。 该项目为学生提供电化学、传感器设计和开发、显微镜、纳米纤维和表征等领域的跨学科培训。电化学成像对于许多应用至关重要，例如对电极/溶液界面的基本理解、催化剂筛选和神经元分泌。以前的方法具有有限的时间分辨率，难以研究随机氧化还原事件。该项目将通过在FEEM系统中开发和利用非常大的电化学微电极阵列来解决这些挑战。实际上，在理想情况下，这种FEEM方法应该能够同时阅读数百万个微电极，以成像瞬态并行氧化还原事件。因此，该项目旨在将FEEM建立为一种有用的分析工具，可以被其他科学家在一系列学科中采用，包括神经化学，环境分析和电催化剂筛选。

With this award, funded by the Chemical Measurement and Imaging Program of the Chemistry Division, Professor Bo Zhang of University of Washington is developing a new analytical microscopy method; namely fluorescence-enabled electrochemical microscopy (FEEM), for imaging transient dynamic redox processes with large electrochemical arrays. A fundamental underlying concept of this research is the conversion of an electrical signal to fluorescence so that optical microscopy can be used to monitor a redox species such as dopamine. This project will develop FEEM as a tool for understanding molecular transport, neuronal activity, and neuron-neuron communication in the brain. The broader impacts are addressed through the recruitment of women and members of underrepresented groups to participate in this science. The project provides interdisciplinary student training in the areas of electrochemistry, sensor design and development, microscopy, and nanofabrication and characterization.Electrochemical imaging is vital for numerous applications such as fundamental understanding of the electrode/solution interface, catalyst screening, and neuronal secretion. Previous methods have limited temporal resolution and are difficult to study stochastic redox events. This project will address these challenges by developing and utilizing very large electrochemical arrays of microelectrodes in FEEM systems. Indeed, in the ideal, this FEEM approach should enable the simultaneous reading of millions of microelectrodes to image transient parallel redox events. As such, this project aims to establish FEEM as a useful analytical tool that could be adopted by other scientists in a range of disciplines including neurochemistry, environmental analysis, and electrocatalyst screening.