Fluorescent Heteroditopic Ligands - Coordination Chemistry, Photophysics, and Sensing Applications

荧光异二位配体 - 配位化学、光物理学和传感应用

• 批准号: 1213574
• 负责人: Lei Zhu
• 金额: $ 36.3万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1213574&HistoricalAwards=false
• 关键词: Fluorescent; Heteroditopic; Ligands; Coordination; Chemistry

With the support of an award from the Chemical Structure, Dynamics and Mechanism Program (CSDM) at the National Science Foundation, Professor Lei Zhu at the Florida State University will lead a research program that investigates the fundamental photophysics and coordination chemistry of fluorescent heteroditopic ligands. This work may lead to the development of fluorescent indicators for quantitative analytical applications using fluorescence microscopic means. The preliminary data from the PI's laboratory introduced the coumarin-based dual-emitting molecules as suitable structural platforms for studying the mechanism of dual emission. In the proposed work, steady state and time-resolved spectroscopic experiments will be used to reveal a kinetic model to account for dual fluorescence. Furthermore, dual fluorescence that is tunable via engineered interactions between electron acceptor moieties and a target molecule in a heteroditopic ligand framework offers opportunities for ratiometric sensor development. The proposed investigations on the fluorescent heteroditopic ligands containing a spirolactam form of rhodamine shall reveal the factors that govern the efficiency of intramolecular electron transfer of spirolactam-containing fluorophores, and the kinetics of spirolactam ring-opening. Resolving these two issues will advance the fundamental understanding on the rhodamine spirolactam chemistry, which provides a sound basis for incorporating rhodamine spirolactam in a sensor structure. This award to Professor Zhu at the Florida State University will lead to results that enrich the knowledge base on the fundamental molecular photophysics and coordination chemistry. Furthermore, tunable dual-fluorescing heteroditopic ligands can be developed into ratiometric fluorescent sensors when a specific interaction between the fluorophore and a target molecule is designed into the fluorophore structure. Investigations on the rhodamine spirolactam chemistry and the metal-coordination dependent ring-opening kinetics will aid further sensor development based on this system with a largest possible signal-to-noise ratio. Some of the broader impacts of the proposed activities derive from the PI's commitment to teaching, and to advocating the dissemination of cutting-edge scientific research to the broader public. With the support of this award, a course on the societal/cultural interests on chemical research to chemistry majors and graduate students will be developed. The chemistry- and graduate education-related contents from motion pictures and chemistry-related blogs will be used as teaching materials for training young science communicators to the public at large.

在美国国家科学基金会化学结构、动力学和机制计划（CSDM）的资助下，佛罗里达州立大学的朱磊教授将领导一个研究计划，研究荧光异位配体的基础物理和配位化学。这项工作可能会导致荧光指示剂的定量分析应用，使用荧光显微镜手段的发展。PI实验室的初步数据介绍了香豆素基双发射分子作为研究双发射机制的合适结构平台。在拟议的工作中，稳态和时间分辨光谱实验将被用来揭示一个动力学模型来解释双荧光。此外，通过电子受体部分和异位配体框架中的靶分子之间的工程化相互作用可调谐的双重荧光为比率传感器开发提供了机会。所提出的调查的荧光heteroditopic配体含有螺内酰胺形式的罗丹明将揭示的因素，支配的效率的分子内电子转移的螺内酰胺含荧光团，和螺内酰胺开环的动力学。解决这两个问题将推进对罗丹明螺内酰胺化学的基本理解，这为将罗丹明螺内酰胺纳入传感器结构提供了良好的基础。此次授予佛罗里达州立大学的朱教授的奖项将导致丰富基础分子物理学和配位化学知识基础的成果。此外，可调的双荧光异位配体可以开发成比率荧光传感器时，荧光团和目标分子之间的特定相互作用被设计成荧光团结构。罗丹明螺内酰胺化学和金属配位依赖的开环动力学的调查将有助于进一步传感器的发展，基于这个系统具有最大可能的信噪比。拟议活动的一些更广泛的影响来自PI对教学的承诺，并倡导向更广泛的公众传播尖端科学研究。在该奖项的支持下，将为化学专业和研究生开发一门关于化学研究的社会/文化兴趣的课程。电影和化学相关博客中与化学和研究生教育相关的内容将被用作向公众培养年轻科学传播者的教材。