A Multi-Spectroscopic Investigation of Protein Structure in Biosilica Composites

生物二氧化硅复合材料中蛋白质结构的多光谱研究

• 批准号: 1219509
• 负责人: Gary Drobny
• 金额: $ 35万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-15 至 2016-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1219509&HistoricalAwards=false
• 关键词: Multi; Spectroscopic; Investigation; Protein; Structure

With this award, the Chemistry of Life Processes Program is funding a collaboration between the solid state NMR (ssNMR) group of Gary Drobny at the University of Washington, Seattle, USA, and the optical spectroscopy group of Tobias Weidner at the Max Planck Institute for Polymer Research, Mainz, Germany. The NSF will be funding the U.S. team and the Deutsche Forshungsgemeinshaft will be funding the German team. This collaboration is aimed at elucidating to high resolution, the structures of proteins and peptides in biosilica composites. Not only will this research provide an atomic level structural picture of how proteins structure when in contact with silica, but the information will be helpful in understanding how biogenic silica is modeled into elaborate macroscopic structures under relatively mild conditions compared to the extreme conditions (high temperatures, pressures etc.) that are involved in the production and manipulation of anthropogenic silica.There are potential long term goal implications of these collaborative studies toward understanding the nature of an important problem in chemical biology; namely peptide-silica interactions and the roles played by peptide secondary, tertiary, and quaternary structure as well as amino acid modifications in controlling the morphology of biosilica composites. These approaches will develop further a set of spectroscopic techniques for structurally characterizing proteins as they exist in other types of biomineral composites. The interdisciplinary and international nature of the collaboration is expected to be beneficial for both the U.S. scientists funded by the NSF and the German scientists funded by the Deutsche Forschungsgemeinshaft.

有了这个奖项，生命过程的化学计划资助之间的固态NMR（ssNMR）组加里Drobny在华盛顿，西雅图，美国大学，和光谱组Tobias Weigeant在马克斯普朗克研究所聚合物研究，美因茨，德国的合作。 美国国家科学基金会将为美国团队提供资金，德国研究基金会将为德国团队提供资金。 这项合作的目的是阐明高分辨率，生物二氧化硅复合材料中的蛋白质和肽的结构。这项研究不仅将提供蛋白质与二氧化硅接触时如何结构的原子水平结构图，而且这些信息将有助于理解生物二氧化硅如何在相对温和的条件下（与极端条件（高温，压力等）相比）建模为精细的宏观结构。这些合作研究对理解化学生物学中一个重要问题的本质具有潜在的长期目标意义;即肽-二氧化硅相互作用和肽二级、三级和四级结构以及氨基酸修饰在控制生物二氧化硅复合材料形态中所起的作用。这些方法将进一步发展一套光谱技术，用于在结构上表征蛋白质，因为它们存在于其他类型的生物矿物复合材料。 该合作的跨学科和国际性质预计将有利于由NSF资助的美国科学家和由Deutsche Forschungsgemeinshaft资助的德国科学家。