MRI: Elucidating Biomechanistic Processes Using Confoncal Microscopy at San Jose State Univerisity

主要研究仪器计划的资金将用于购买和支持圣何塞州立大学的蔡司LSM 700共聚焦显微镜。 来自生物、化学和土木工程系的研究小组以及圣克拉拉大学的生物学教授将成为主要用户。 一个共同的主题，研究，将启用和加强这一收购是阐明生物力学过程。 在遗传学，发展，环境微生物学，细胞生物学，免疫学，生物化学和土木工程领域的专业知识的教师将包括共聚焦显微镜的主要用户。 具体而言，以下研究项目将利用这种显微镜：凋亡诱导的r-去整合素;分子机制，介导神经元电路的形成和维护;机制，乙醇暴露影响突触可塑性;表征新的未培养的细菌群体的生态生理学研究;免疫调节淋巴细胞运输;细胞定位的肌醇聚糖;检查小鼠卵巢发育过程中的血管生成，1，25-二羟基维生素D3 [1，25（OH）2D 3]与核维生素D受体（VDR）结合产生的信号转导;使用微生物测年和共聚焦显微镜测定结构裂缝;圣何塞州立大学是北方加州的一所少数民族服务机构，有很大比例的学生有身体残疾。 研究小组积极纳入本科研究人员和学生在硕士？的水平在他们的项目。 该大学培训基础设施的四个主要组成部分将受到购买共聚焦显微镜的影响：1）增加本科生参与研究，2）促进主动学习，3）专业硕士课程的学生培训，以及4）与其他机构的教师和学生合作。将利用这种共聚焦显微镜的教师是几个项目的研究导师，这些项目旨在增加攻读博士学位的少数民族学生的数量。科学学位，包括NIH-MARC，MBRS-RISE，HHMI-SCRIBE和NSF-REU RUMBA。 学习在他们的研究中使用这种先进的显微镜将有助于学生为科学事业做好准备。 此外，从这台显微镜收集的数据将用于本科课程，将当代研究带入课堂。 最后，包括圣克拉拉大学在内的周边社区的小型机构将有机会利用这种显微镜来增加他们的研究潜力。

Funds from this Major Research Instrumentation Program grant will be used for the purchase and support of a Zeiss LSM 700 Confocal Microscope at San José State University. Research groups from the Biology, Chemistry and Civil Engineering departments will be among the major users, as well as a biology professor from Santa Clara University. A common theme to the research that will be enabled and enhanced by this acquisition is to elucidate biomechanistic processes. Faculty members with expertise in the areas of genetics, development, environmental microbiology, cell biology, immunology, biochemistry, and civil engineering will comprise the major users of the confocal microscope. Specifically, the following research projects will utilize this microscope: apoptotic induction by r-disintegrins; molecular mechanisms that mediate neuronal circuit formation and maintenance; mechanisms by which ethanol exposure affects synaptic plasticity; characterization of novel uncultured bacterial groups for ecophysiological studies; immunomodulation of lymphocyte trafficking; cellular localization of inositol glycans; examining angiogenesis during ovarian development in mice, signal transduction resulting from binding of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] to the nuclear vitamin D receptor (VDR); using microbiological dating and confocal microscopy to date structural cracks; and developmental analysis of LIN-1 expression.San José State University is a minority serving institution in Northern California with a large proportion of students with physical disabilities. Research groups actively incorporate undergraduate researchers and students at the Master?s level in their projects. Four main components of the training infrastructure at the university will be impacted by the purchase of this confocal microscope: 1) increased involvement of undergraduate students in research, 2) promoting active learning, 3) student training in specialized masters programs, and 4) collaborations with faculty and students at other institutions. Faculty that will utilize this confocal microscope are research mentors for several programs that aim to increase the number of minority students who pursue Ph.D. degrees in the sciences, including NIH-MARC, MBRS-RISE, HHMI-SCRIBE, and NSF-REU RUMBA. Learning to use this state of the art microscope in their research will help prepare students for careers in science. In addition, data collected from this microscope will be used in undergraduate courses to bring contemporary research into the classroom. Finally, smaller institutions in the surrounding community including Santa Clara University will have the opportunity to utilize this microscopy to increase their research potential.