MRI: Elucidating Biomechanistic Processes Using Confoncal Microscopy at San Jose State Univerisity

MRI：在圣何塞州立大学使用共聚焦显微镜阐明生物力学过程

• 批准号: 1229817
• 负责人: Rachael French
• 金额: $ 33.68万
• 依托单位: San Jose State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229817&HistoricalAwards=false
• 关键词: MRI; Elucidating; Biomechanistic; Processes; Using

Funds from this Major Research Instrumentation Program grant will be used for the purchase and support of a Zeiss LSM 700 Confocal Microscope at San José State University. Research groups from the Biology, Chemistry and Civil Engineering departments will be among the major users, as well as a biology professor from Santa Clara University. A common theme to the research that will be enabled and enhanced by this acquisition is to elucidate biomechanistic processes. Faculty members with expertise in the areas of genetics, development, environmental microbiology, cell biology, immunology, biochemistry, and civil engineering will comprise the major users of the confocal microscope. Specifically, the following research projects will utilize this microscope: apoptotic induction by r-disintegrins; molecular mechanisms that mediate neuronal circuit formation and maintenance; mechanisms by which ethanol exposure affects synaptic plasticity; characterization of novel uncultured bacterial groups for ecophysiological studies; immunomodulation of lymphocyte trafficking; cellular localization of inositol glycans; examining angiogenesis during ovarian development in mice, signal transduction resulting from binding of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] to the nuclear vitamin D receptor (VDR); using microbiological dating and confocal microscopy to date structural cracks; and developmental analysis of LIN-1 expression.San José State University is a minority serving institution in Northern California with a large proportion of students with physical disabilities. Research groups actively incorporate undergraduate researchers and students at the Master?s level in their projects. Four main components of the training infrastructure at the university will be impacted by the purchase of this confocal microscope: 1) increased involvement of undergraduate students in research, 2) promoting active learning, 3) student training in specialized masters programs, and 4) collaborations with faculty and students at other institutions. Faculty that will utilize this confocal microscope are research mentors for several programs that aim to increase the number of minority students who pursue Ph.D. degrees in the sciences, including NIH-MARC, MBRS-RISE, HHMI-SCRIBE, and NSF-REU RUMBA. Learning to use this state of the art microscope in their research will help prepare students for careers in science. In addition, data collected from this microscope will be used in undergraduate courses to bring contemporary research into the classroom. Finally, smaller institutions in the surrounding community including Santa Clara University will have the opportunity to utilize this microscopy to increase their research potential.

这项重大研究仪器计划赠款的资金将用于购买和支持圣何塞州立大学的蔡司 LSM 700 共焦显微镜。 来自生物、化学和土木工程系的研究小组以及圣克拉拉大学的生物学教授将成为主要用户。 此次收购将实现和加强的研究的一个共同主题是阐明生物力学过程。 具有遗传学、发育、环境微生物学、细胞生物学、免疫学、生物化学和土木工程领域专业知识的教师将成为共聚焦显微镜的主要用户。 具体来说，以下研究项目将利用该显微镜：r-解整合素诱导细胞凋亡；介导神经元回路形成和维持的分子机制；乙醇暴露影响突触可塑性的机制；用于生态生理学研究的新型未培养细菌群的表征；淋巴细胞运输的免疫调节；肌醇聚糖的细胞定位；检查小鼠卵巢发育过程中的血管生成，以及 1,25-二羟基维生素 D3 [1,25(OH)2D3] 与核维生素 D 受体 (VDR) 结合产生的信号转导；使用微生物测年和共焦显微镜来确定结构裂缝的日期； LIN-1表达的发育分析。圣何塞州立大学是北加州的一所少数族裔服务机构，有很大比例的身体残疾学生。 研究小组积极地将本科生研究人员和硕士水平的学生纳入他们的项目中。 购买这款共焦显微镜将影响大学培训基础设施的四个主要组成部分：1）增加本科生对研究的参与，2）促进主动学习，3）专业硕士课程的学生培训，以及4）与其他机构的教师和学生的合作。将使用这种共焦显微镜的教师是多个项目的研究导师，这些项目旨在增加攻读博士学位的少数族裔学生的数量。科学学位，包括 NIH-MARC、MBRS-RISE、HHMI-SCRIBE 和 NSF-REU RUMBA。 学习在研究中使用这种最先进的显微镜将有助于学生为科学职业做好准备。 此外，从该显微镜收集的数据将用于本科课程，将当代研究带入课堂。 最后，包括圣克拉拉大学在内的周边社区的小型机构将有机会利用这种显微镜来增加其研究潜力。