Research Starter Grant: Investigating the impacts of loline catabolizing bacteria on fitness of their tall fescue host

研究启动资助：调查黑麦草分解代谢细菌对其高羊茅宿主健康的影响

• 批准号: 1237624
• 负责人: Elizabeth Roberts
• 金额: $ 5万
• 依托单位: Southern Connecticut State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1237624&HistoricalAwards=false
• 关键词: Research; Starter; Grant; Investigating; impacts

Loline alkaloids are made by the endophytic fungus Neotyphodium sp. as part of a symbiotic relationship to keep insect herbivores from consuming their tall fescue host. Previous research has indicated that some epiphytic bacterial colonizers of tall fescue can use these alkaloids as a sole carbon source. Such bacteria grow to higher population sizes on loline containing grasses than other bacterial strains. It is thought that a multi-trophic symbiosis is occurring on the grass phyllosphere between the loline catabolizing bacteria, the host, and the endophytic fungus. This project will test the potential positive impact of loline catabolizing bacterial epiphytes on growth of the host. The bacteria will be screened for production of the plant growth regulators, auxin and cytokinin, through growth assays, microscopy, and biochemistry. Additionally, the protective impacts of loline catabolizing bacteria on the host grasses will be investigated by screening them for the antimicrobial production. The ability of the bacteria to protect their hosts via niche exclusion will also be examined. A combination of culturing and biochemistry techniques will be conducted for these tests. Finally, the potential to take advantage of the affinity of loline catabolizing strains to Neotyphodium sp. harboring grasses for the bioremediation of the major pollutant Atrazine will be tested. The data collected from this work will have broad implications on the current scientific knowledge concerning microbe:microbe interactions. Potentially, this work may result in an efficient way to remove atrazine from the environment, which would generate safer ground water for future generations.

loline生物碱是由内生真菌新近肾上腺素sp。制造的。作为共生关系的一部分，以防止食草动物消耗其高大的羊茅宿主。先前的研究表明，高羊茅的一些附生细菌菌落可以将这些生物碱用作唯一的碳源。与其他细菌菌株相比，这种细菌在含有草的萝卜含量上生长到更高的人口大小。人们认为，在洛琳（Loline）分解代谢细菌，宿主和内生真菌之间的草层上发生了多营养性共生。该项目将测试可分解细菌附生植物对宿主生长的潜在积极影响。通过生长测定，显微镜和生物化学，将筛选细菌以生产植物生长调节剂，生长素和细胞分裂素。此外，通过筛查抗菌生产的质量，将研究它们对宿主草的保护作用对宿主草的保护作用。还将检查细菌通过利基排除保护其宿主的能力。将对这些测试进行培养和生物化学技术的结合。最后，利用萝卜质分解代谢菌株的亲和力的潜力。将测试含有主要污染物阿特拉津的生物修复草的草。从这项工作中收集的数据将对有关微生物的当前科学知识具有广泛的影响：微生物相互作用。这项工作可能会导致一种有效的方法使从环境中消除阿atrazine，这将为子孙后代带来更安全的地下水。