Research Starter Grant: Metabolic Pathways in Bacterial Utilization of Hemicellulosic Sugars

研究启动资金：细菌利用半纤维素糖的代谢途径

• 批准号: 1337292
• 负责人: Ludmilla Aristilde
• 金额: $ 5万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337292&HistoricalAwards=false
• 关键词: Research; Starter; Grant; Metabolic; Pathways

Intellectual MeritAs part of the cycle of carbon on earth, plants store carbon dioxide into their biomass as organic carbon, which can be subsequently respired back into the atmosphere as carbon dioxide via microbial degradation of plant detritus. Natural microbial respiration of plant biomass is estimated to contribute eight to ten times greater carbon dioxide inputs than human activities. Previous field observations have indicated a dependence of decomposition of plant matter on the type of vegetation cover and that an increase in temperatures can lead to increased decomposition rates. Thus, of important interest is a fundamental understanding of the biological factors controlling the decomposition rates of different plant matter components. However, the biological pathways responsible for the respiration of plant matter components are not well understood. The working hypothesis to be addressed by this research is that the decomposition rates of plant matter are tightly linked to the pathways underlying the catabolism of the different organic moieties in plant matter. Using Pseudomona putida as a model aerobic bacterial organism, the pathways by which hemicellulosic sugars are metabolized will be investigated. The results from this study will contribute to our understanding of the biochemical dynamics underlying the metabolism of plant matter, a necessary pre-requisite to accurate predictions of the feedback response of the global carbon cycle to climate change. Broader ImpactsThis project will involve the training and participation of young scientists to address global environmental issues at a fundamental level. Given the global importance of this research with environmental implications, the obtained results will be of interest to scientists across various biological and chemical disciplines and to society at-large, and will be published in journals with broad dissemination.

智慧价值作为地球碳循环的一部分，植物将二氧化碳以有机碳的形式储存在它们的生物量中，然后通过微生物对植物碎屑的降解，这些有机碳可以作为二氧化碳呼吸回到大气中。据估计，植物生物量的自然微生物呼吸对二氧化碳输入的贡献是人类活动的八到十倍。以前的实地观测表明，植物物质的分解依赖于植被复盖的类型，温度的升高会导致分解速率的增加。因此，重要的是对控制不同植物物质成分分解速率的生物因素有一个基本的了解。然而，负责植物物质成分呼吸的生物途径还不是很清楚。这项研究要解决的工作假设是，植物物质的分解速度与植物物质中不同有机部分的分解代谢途径密切相关。以恶臭假单胞菌为模式好氧细菌，研究半纤维素糖的代谢途径。这项研究的结果将有助于我们理解植物物质代谢的生化动力学，这是准确预测全球碳循环对气候变化的反馈响应的必要前提。更广泛的影响该项目将涉及青年科学家的培训和参与，以从根本上解决全球环境问题。鉴于这项具有环境影响的研究的全球重要性，所获得的结果将引起不同生物和化学学科的科学家以及整个社会的兴趣，并将在广泛传播的期刊上发表。

项目成果

Structural characterization of multiple pyoverdines secreted by two Pseudomonas strains using liquid chromatography-high resolution tandem mass spectrometry with varying dissociation energies

• DOI: 10.1007/s00216-015-8659-5
• 发表时间: 2015-06-01
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Wei, Hua;Aristilde, Ludmilla
• 通讯作者: Aristilde, Ludmilla