In depth characterization of organelle tubulation phenomenon using plastid stromules

荧光蛋白标记的细胞区室的生命细胞成像显示，细胞器显示出灵活的形态，可以在几秒钟内发生显着变化。线粒体、过氧化物酶体和质体共同的形态学主题是管状膜延伸的形成。虽然它们的形成似乎受到严格的监管，我们目前的知识是非常有限的，他们的作用，细胞的活力，几乎没有什么是已知的细胞过程中，这些小管参与。以下的想法，这些结构的功能，可以阐明的过程中，他们参与的识别，我筛选在我的博士学位，专注于质体，质体小管（基质）相关的刺激。我发现葡萄糖和蔗糖是特定的诱导子座形成，表明连接的子座碳水化合物metabolisation.I的目的是进一步研究建议的连接的子座和碳水化合物代谢的拟南芥突变体和非代谢糖类似物的使用。此外，我将测试条件下，转录概况是现成的，为他们的小胚相关性。通过这一点，我将解决的遗传背景的子实体形成和EMS诱变筛选将补充遗传方法。下一步，我将把研究范围扩大到线粒体和过氧化物酶体，并将评估与子座相关的刺激对这两种细胞器的影响，这一项目最终将不仅有助于阐明子座形成的遗传背景，而且有助于将子座置于植物细胞的生理和代谢背景中。通过将范围扩大到线粒体和过氧化物酶体，我提出的工作有可能告诉我们真核细胞维持最佳亚细胞环境的机制，尽管不断受到许多环境压力的挑战。

Life cell imaging of fluorescent protein labeled cell compartments revealed that organelles show a flexible morphology, which can change dramatically within seconds. A morphologic theme common to mitochondria, peroxisomes and plastids is the formation of tubular membrane extensions. Although their formation appears to be tightly regulated our current knowledge about their role for cell viability is very limited and hardly anything is known about cellular processes these tubules are involved.Following the idea that the function of these structures can be elucidated by the identification of processes they are involved in, I screened during my PHD, focusing on plastids, for plastid tubule (stromule) relevant stimuli. I found glucose and sucrose to be specific inducers of stromule formation, suggesting a connection of stromules to carbohydrate metabolism.I aim to further investigate the suggested connection of stromules and carbohydrate metabolism by the use of Arabidopsis thaliana mutants and non-metabolizable sugar analogues. Furthermore I will test conditions for which transcription profiles are readily available for their stromule relevance. By this and I will address the genetic background of stromule formation and an EMS mutagenesis screen will complement the genetic approach. In a next step I will expand the scope to mitochondria and peroxisomes and will evaluate the effect of stromule relevant stimuli on these two organelles.This project ultimately will help not only to clarify the genetic background of stromule formation, but will also help to place stromules in the physiological and metabolic context of the plant cell. By expanding the scope to mitochondria and peroxisomes my proposed work has the potential of informing us about the mechanisms employed by eukaryotic cells for maintaining an optimal subcellular milieu despite being challenged constantly by numerous environmental stresses.