Comparative cell-lineage analysis in Deuterostomia

后口动物中的比较细胞谱系分析

• 批准号: 208436799
• 负责人: Dr. Thomas Stach
• 金额: --
• 依托单位: Forschungsgruppe Elektronenmikroskopie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/208436799?language=en
• 关键词: Comparative; cell; lineage; analysis; Deuterostomia

The phylogeny of deuterostomes and evolution of chordate features is controversially debated. Molecular phylogenies result in conflicting topologies and morphological characters and lesser animal taxa are often ignored. However, morphology hosts a plethora of informative characters and is currently transformed through internet-based databases, collaborative ontologies, and new standards in data documentation and character coding. In the present proposal, I request funding to further contribute to this endeavor, focusing on comparative ontogenetic cell-lineage analyses in deuterostomes. Precise cell-lineage data gathered by 4D-microscopy have been successfully used in an exemplary study of the tunicate Oikopleura dioica (Stach et al., 2008) and confirmed their usefulness in phylogenetic studies. Here, I specifically propose to comparatively investigate the cell-lineage of several ascidian species, a cephalochordate, and an echinoderm. This will significantly broaden our conception of the evolution of development at the cellular level and of morphogenesis within deuterostomes. By utilizing the resulting data in collaboratively developed phylogenetic matrices that are integrated in the online-platform Morph-D-Base, the contribution of deuterostome morphology to metazoan phylogenies will be considerably advanced.

后口动物的系统发育和脊索动物特征的进化是有争议的。分子系统发育导致相互矛盾的拓扑和形态特征，较小的动物类群往往被忽略。然而，形态学承载了大量的信息字符，目前通过基于互联网的数据库、协作本体以及数据文档和字符编码的新标准进行了转换。在目前的提案中，我请求进一步资助这一努力，重点是对后口动物的比较个体发生细胞谱系分析。通过4d显微镜收集的精确细胞谱系数据已成功地用于对被囊动物Oikopleura dioica的示范研究（Stach等人，2008），并证实了它们在系统发育研究中的有用性。在这里，我特别建议比较研究几种海鞘、一种头脊索动物和一种棘皮动物的细胞系。这将大大拓宽我们对细胞水平上的发育进化和后口动物形态发生的概念。通过利用整合在在线平台morphd - base中的合作开发的系统发育矩阵中的结果数据，后口动物形态学对后生动物系统发育的贡献将大大提高。