Nanoscaled Architectures for highly Sensitive Biosensing of Small molecules

该项目结合先进的生物分子技术、化学和纳米技术，旨在研制超灵敏和特异性的免疫传感器，用于检测复杂环境中的重点污染物。它依赖于四个相互依存的任务：1)针对多环芳烃（苯并[a]芘）、药物（双氯芬酸）和藻类毒素（微囊藻毒素）的高仿射单克隆抗体的生产和详细表征。2)表面的纳米结构作为结合分子探针的合适平台。3)利用金纳米粒子及其优化设计，提高光学检测技术（表面等离子体共振、SPR、表面增强拉曼散射、SERS和化学发光读取）的灵敏度。4)使用带有耗散测量（QCM-D）的石英晶体微天平以及SPR和SERS进行测试开发，目的是达到上述污染物在低ng/ l范围内的检测限。同时，将开发一种新的基于微阵列的自动流式ELISA。所有测试将使用实际样品和LC-MS进行验证。该项目的成功依赖于相关合作伙伴、生物化学家、分子化学家、表面科学家的专业知识互补，以及他们之间的不断交流。合作伙伴2将为其他人提供新的仿射抗体，并将开发多分析物测试。合作伙伴1将专注于表面纳米结构以及SPR， SERS和QCM的所有检测测量。合作伙伴3将设计和合成新的分子结构（功能化多金属氧酸盐），产生一种非常新颖的表面化学图案。这个项目雄心勃勃，而且可能有风险，因为每一步都有新的发展；由于已经取得了初步结果，这也是非常有希望的。预期在基础和应用层面都能取得成果。毫无疑问，在高影响力的科学期刊上发表文章是可以预期的。目标技术在与健康和环境保护有关的各种应用领域也会引起相当大的兴趣。

Combining advanced biomolecular technology, chemistry and nanotechnology, this project aims at the elaboration of ultra-sensitive and specific immunosensors for the detection of priority pollutants in complex environments. It relies on four interdependent tasks: 1) Production and detailed characterization of high-affine monoclonal antibodies towards polycyclic aromatic hydrocarbons (benzo[a]pyrene), pharmaceuticals (diclofenac) and algae toxins (microcystins). 2) Nanostructuring of surfaces to serve as appropriate platforms for binding molecular probes. 3) Use of gold nanoparticles, and their optimized design, to enhance the sensitivity of optical detection techniques (Surface Plasmon Resonance, SPR, Surface Enhanced Raman Scattering, SERS, and chemiluminescence readout). 4) Test development using Quartz Cristal Microbalance with dissipation measurements (QCM-D) as well as SPR and SERS with the objective of reaching detection limits in the low ng/L-range for the above cited pollutants. In parallel, a new automated microarray-based flow-through ELISA will be developed. All tests will be validated using real samples and LC-MS. The success of this project relies on the complementary expertise of the involved partners, biochemists, molecular chemists, surface scientists, and on constant exchanges between them. Partner 2 will provide others with new affine antibodies, and will develop the multi-analyte test. Partner 1 will focus on surface nanostructuration as well as on all the detection measurements by SPR, SERS and QCM. Partner 3 will design and synthesize new molecular architectures (functionalized polyoxometalates), yielding a very original way of surface chemical patterning. This project is ambitious and likely risky in the sense that each step includes new developments; it is also very promising thanks to already obtained preliminary results. Results are expected both at fundamental and applied levels. Publications in high impact scientific journals can be undoubtedly expected. The aimed technology would also be of considerable interest in various applied domains linked to health and environmental protection.