A novel biosynthetic pathway for the unique volatile 'sodorifen' of Serratia odorifera 4Rx13

沙雷氏菌 4Rx13 独特的挥发性“sodorifen”的新型生物合成途径

• 批准号: 209316534
• 负责人: Professorin Dr. Birgit Piechulla
• 金额: --
• 依托单位: Abteilung Biochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/209316534?language=en
• 关键词: novel; biosynthetic; pathway; unique; volatile

Volatiles are well-established inter and intra species communication and recognition signals. Only recently it was demonstrated that beside other organisms also bacteria emit a wealth of volatiles. Particularly rich is the blend of Serratia odorifera 4Rx13 (up to 100 volatiles). The structure of the dominant compound (C16H32, 1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo[3.2.1]oct-6-ene, named sodorifen) is unique and novel to science. The polymethylated structure is extremely unusual and the biosynthetic pathway is completely unknown and presently not predictable. The following strategies will be performed to elucidate the biosynthetic pathway of this new natural compound: i) Feeding experiments indicated met, thr, ala, his, lys, or succinate and fumarate as favored substrates for the biosynthesis of sodorifen. Subsequently 13Camino acids and 13C-succinate, and NMR will be performed to predict the composition of sodorifen and the underlying reactions. ii) Proteomes of sodorifen emitting and non-emitting Serratia species should allow the detection of sodorifen biosynthesis enzymes. iii) The genome of S. odorifera 4Rx13 was completely sequenced to support the identification of putative biosynthesis genes. Newly isolated genes will be used a) to functionally characterize them via knockout mutants and b) to search for the origin of the biosynthetic pathway in metagenomic libraries. Ultimately research concerning the biological/ecological relevance of sodorifen will be initiated.

挥发物是物种间和物种内的通信和识别信号。直到最近才证明，除了其他生物体之外，细菌也会释放出大量的挥发物。特别丰富的是气味沙雷氏菌4 Rx 13（多达100种挥发物）的混合物。主要化合物（C16 H32，1，2，4，5，6，7，8-七甲基-3-亚甲基双环[3.2.1]辛-6-烯，命名为sodorifen）的结构是科学上独特和新颖的。多甲基化的结构是非常不寻常的，生物合成途径是完全未知的，目前无法预测。将执行以下策略来阐明这种新的天然化合物的生物合成途径：i）饲养实验表明，蛋氨酸、苏氨酸、丙氨酸、组氨酸、赖氨酸或琥珀酸盐和富马酸盐是钠草醚生物合成的有利底物。随后，将进行13 C-氨基酸和13 C-琥珀酸酯以及NMR以预测钠草醚的组成和潜在的反应。ii）散发钠草醚和不散发钠草醚的沙雷氏菌属物种的蛋白质组应允许检测钠草醚生物合成酶。iii）S.为了支持推定的生物合成基因的鉴定，对气味植物4 Rx 13进行了完全测序。新分离的基因将用于a）通过敲除突变体对其进行功能表征，和B）在宏基因组文库中搜索生物合成途径的起源。最终将启动关于sodorifen生物/生态相关性的研究。