CAREER: Elucidating Surfactin Modus Operandi with 2D IR Spectroscopy

职业：利用 2D 红外光谱阐明表面活性素的操作方式

• 批准号: 1255658
• 负责人: Amber Krummel
• 金额: $ 60万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1255658&HistoricalAwards=false
• 关键词: CAREER; Elucidating; Surfactin; Modus; Operandi

Through this NSF CAREER award, funded by the Chemical Structure, Dynamics, and Mechanisms B Program (CSDM-B), Professor Amber T. Krummel from Colorado State University will identify the means by which a small pore-forming toxin, surfactin, will interact with model lipid membranes using two-dimensional infrared (2D IR) spectroscopy. Surfactin is known to have antibacterial, antiviral, and antitumor activity, but surfactin has also been implicated in cell-signaling activities during biofilm formation. This project will identify the differences in the mode of action by surfactin during these different activities. The structural sensitivity offered by 2D IR spectroscopy will be utilized to quantify the molecular details of the surfactin-lipid interaction during the surfactin insertion and lipid membrane disruption processes.Pore-forming toxins are proteins that are synthesized by many organisms, including the human immune system. These proteins insert into cell membranes in order to allow small molecules to move into cells, cause cell leakage, or to disrupt the integrity of cells such that cell death occurs. In this work, advanced optical spectroscopy techniques will be used to monitor the protein-lipid interaction with structural sensitivity. The results from these investigations will lead to a new understanding of how a small pore-forming toxin, surfactin, self-assembles and interacts with lipids in order to drive cell signaling processes and the disruption of lipid membranes. During the course of this project, undergraduate researchers and graduate student researchers will be trained in advanced optical spectroscopy techniques, chemical modeling, and data analysis. The approaches used to train the student researchers involved with this project will also be incorporated into the undergraduate curriculum for Chemistry Majors at Colorado State University. Thus, this project will generate new insights into an important chemical system and it will generate new approaches to training the next generation of Chemistry majors at Colorado State University.

通过这个NSF职业奖，由化学结构，动力学和机制B计划（CSDM-B）资助，教授琥珀T。来自科罗拉多州立大学的Krummel将使用二维红外光谱法鉴定一种小的成孔毒素表面活性素与模型脂质膜相互作用的方式。 已知表面活性素具有抗菌、抗病毒和抗肿瘤活性，但表面活性素还涉及生物膜形成期间的细胞信号传导活性。 本项目将确定在这些不同的活动中，表面活性素的作用模式的差异。 二维红外光谱提供的结构灵敏度将被用来量化表面活性素插入和脂质膜破坏过程中表面活性素-脂质相互作用的分子细节。成孔毒素是由许多生物体合成的蛋白质，包括人类免疫系统。 这些蛋白质插入细胞膜，以允许小分子进入细胞，导致细胞渗漏，或破坏细胞的完整性，从而发生细胞死亡。 在这项工作中，先进的光学光谱技术将用于监测蛋白质-脂质相互作用的结构灵敏度。 从这些调查的结果将导致一个新的理解如何形成小孔的毒素，表面活性素，自我组装和与脂质相互作用，以驱动细胞信号传导过程和脂质膜的破坏。 在这个项目的过程中，本科生研究人员和研究生研究人员将接受先进的光谱技术，化学建模和数据分析的培训。 用于培训参与该项目的学生研究人员的方法也将纳入科罗拉多州立大学化学专业的本科课程。 因此，该项目将产生一个重要的化学系统的新见解，它将产生新的方法来培养下一代的化学专业在科罗拉多州立大学。