ABR: Novel Glycoprotein Hormone, Signaling Mechanisms, and Transcriptome Profiling in a Basal Vertebrate

ABR：基础脊椎动物的新型糖蛋白激素、信号机制和转录组分析

• 批准号: 1257476
• 负责人: Stacia Sower
• 金额: $ 62万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-15 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1257476&HistoricalAwards=false
• 关键词: ABR; Novel; Glycoprotein; Hormone; Signaling

This research will investigate the brain and pituitary glycoprotein hormones and respective receptors that regulate reproduction in a the basal vertebrate, the sea lamprey. Lampreys have an ancestral unique pituitary glycoprotein hormone (GpH) not found in any other vertebrate. This project will examine the origin and/or divergence of the pituitary glycoprotein-hormone-receptor families and the molecular mechanisms and dynamics of pituitary GpH signaling. Results will tell whether these hormones share common functional and developmental features with the other hormones found in all other vertebrates. This project will provide key information in neuroscience, neuroendocrinology, molecular biology and vertebrate biology, and it will provide training oppportunities for postdocs, undergraduates and graduate students in these areas. Gaining a further understanding of reproductive hormones and their respective receptors, interactions with neurotransmitters and testing analogs will be critical for development of novel strategies for improving and controlling reproduction. The PI as Director of the Center for Molecular and Comparative Endocrinology (CME) facilitates worldwide and local collaborations, laboratory exchange visits and chalk talks that yield benefits leading to increased student/postdoc engagement in the research community. Outreach activities are varied and broad, including public talks and collaborating with a Biotechnology Program at a local community college. A lamprey brain atlas will also be developed as an interactive neuroanatomy digital resource to be available for the scientific community. The PI and members of the laboratory are involved with an international group of 47 scientists who are annotating the lamprey genome. The data generated through the research will consist of nucleotide and protein sequences, phylogenetic analysis, graphs, three-dimensional structures of the receptors, reconstructed microscopic images, photographs, and hand-recorded observations, and will be made available to qualified parties by the PI so long as such a request does not compromise intellectual property interests, interfere with publication, invade subject privacy, or betray confidentiality.

这项研究将研究大脑和垂体糖蛋白激素以及调节基础脊椎动物（海七鳃鳗）繁殖的相应受体。七鳃鳗拥有一种在任何其他脊椎动物中都没有的祖先独特的垂体糖蛋白激素（GpH）。该项目将研究垂体糖蛋白激素受体家族的起源和/或分歧以及垂体 GpH 信号传导的分子机制和动力学。结果将表明这些激素是否与所有其他脊椎动物中发现的其他激素具有共同的功能和发育特征。该项目将提供神经科学、神经内分泌学、分子生物学和脊椎动物生物学方面的关键信息，并将为这些领域的博士后、本科生和研究生提供培训机会。进一步了解生殖激素及其各自的受体、与神经递质的相互作用以及测试类似物对于开发改善和控制生殖的新策略至关重要。 PI 作为分子和比较内分泌学中心 (CME) 主任，促进全球和地方合作、实验室交流访问和粉笔演讲，从而提高学生/博士后在研究社区的参与度。外展活动多种多样且广泛，包括公开演讲以及与当地社区大学的生物技术项目合作。七鳃鳗脑图谱也将被开发为交互式神经解剖学数字资源，供科学界使用。 PI 和实验室成员与一个由 47 名科学家组成的国际小组一起对七鳃鳗基因组进行注释。研究产生的数据将包括核苷酸和蛋白质序列、系统发育分析、图表、受体的三维结构、重建的显微图像、照片和手工记录的观察结果，只要不损害知识产权利益、干扰出版、侵犯受试者隐私或泄露机密，PI将向合格方提供这些数据。