Reciprocal regulation between the circadian clock and the iron homeostasis network in Arabidopsis

C·Robertson McClungIOS 1257722拟南芥生物钟和铁稳态网络之间的相互调节这个项目将有助于理解植物铁稳态的昼夜调节作用，这一点很重要，原因有两个。首先，铁常常限制植物生长和农业产量。第二，虽然大多数人从植物中获取铁，但植物性饮食是铁的贫乏来源，缺铁是人类最常见的营养失调，影响到世界上大约一半的人口。生物钟整合了时间信息，协调了生物学的许多方面，包括新陈代谢和对压力的反应。具体地说，植物生物钟调节铁稳态基因网络，反过来，铁状态调节时钟功能。这个项目应该有助于形成铁稳态在时间上的完整图景；目标是了解负责整合铁状态信息的基因网络，并协调时间和空间调节的协调反应。从植物生产力和养分含量的角度来看，这应该会改变对作物产量可持续改善的努力。该项目将检验这样一种假设，即昼夜节律功能受损对铁稳态产生不利影响，从而导致适合性降低，在农业环境中，还会导致粮食产量和营养质量下降。了解铁稳态的昼夜规律可能有助于提高作物生产力和食物的营养质量，特别是在广泛的纬度范围内种植的作物。该项目将与蒙特郡科学博物馆合作，在佛蒙特州和新罕布夏州的中学课堂上实施遗传学教学模块。遗传资源(突变品系和构建体)将保存在拟南芥生物资源中心。分子数据将存放在基因表达总览(GEO)数据库中。

C. Robertson McClungIOS 1257722 Reciprocal regulation between the circadian clock and the iron homeostasis network in ArabidopsisThis project will contribute to understanding the role of circadian regulation of iron homeostasis in plants, which is important for two reasons. First, iron often limits plant growth and agricultural yield. Second, although most people acquire iron from plant sources, plant-based diets are poor sources of iron, and iron deficiency represents the single most common human nutritional disorder, affecting approximately one-half of the world's population. The circadian clock integrates temporal information and coordinates many aspects of biology, including metabolism and responses to stress. Specifically, the plant circadian clock regulates the iron homeostasis gene network and, in turn, iron status modulates clock function. This project should help to shape a temporally integrated picture of iron homeostasis; the goal is to understand the network of genes responsible for integrating information about iron status and orchestrating a coordinated response that is temporally as well as spatially regulated. This should transform efforts towards sustainable improvements of crop yields in terms of plant productivity and nutrient content. The project will test the hypothesis that impaired circadian function adversely affects iron homeostasis and thus contributes to reduced fitness and, in agricultural settings, to reduced yield and reduced nutritional quality of food. Understanding the circadian regulation of iron homeostasis may contribute to crop productivity and to nutritional quality of food, particularly in crops grown over broad latitudinal ranges.This project will implement Genetics teaching modules in middle school classrooms in Vermont and New Hampshire in collaboration with the Montshire Museum of Science. Genetic resources (mutant lines and constructs) will be deposited with the Arabidopsis Biological Resource Center. Molecular data will be deposited in the Gene Expression Omnibus (GEO) database.