MAP kinase signaling in the Arabidopsis embryo

拟南芥胚胎中的 MAP 激酶信号传导

• 批准号: 1257805
• 负责人: Wolfgang Lukowitz
• 金额: $ 50.8万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1257805&HistoricalAwards=false
• 关键词: MAP; kinase; signaling; Arabidopsis; embryo

Vascular plants have two fundamentally different axes of growth, roots and shoots, which first become established through precisely orchestrated cell fate decisions in the early embryo. While the mechanisms underlying this patterning process remain poorly understood overall, previous work has clearly identified a key role for the YODA (YDA) cellular signaling pathway. The YDA pathway, which contains a mitogen-activated protein (MAP) kinase module, becomes activated upon fertilization and promotes division of the zygote into a large basal and a small apical daughter. This asymmetric first division defines polarity and sets the stage for subsequent elaboration of the main axis with root and shoot poles. In this project, the molecular mechanisms of YDA signaling in the zygote and early embryo will be analyzed using a diverse set of methodologies, including classical genetics, comparative phylogeny, molecular biology, genomics, microscopy, and biochemistry. Particular attention is dedicated to factors regulating the core MAP kinase module in the context of fertilization, as well as the gene expression programs targeted by the pathway and executing the establishment of polarity. The expected results will make pioneering contributions to our understanding of how the basic body plan of plants is laid out and may inform targeted approaches for initiating embryos from somatic cells in culture.The project will lead to training of a post doctoral associate and a graduate student in plant genetics, molecular biology, genomics, and advanced microscopy. Participation of a high school teacher, high school students, and undergraduate students is an integral part of the research plan; recruitment will be through the Georgia Intern-Fellowships for Teachers (GIFT) program, the Empowered Youth Program (EPY), the University's Summer Undergraduate Research Program (SURP), and the NSF-sponsored Peach State Louis Stokes Alliance for Minority Participation (PSLSAMP).

维管植物有两个根本不同的生长轴，根和芽，它们首先通过早期胚胎中精确协调的细胞命运决定而建立。 虽然这种模式化过程的机制总体上仍然知之甚少，但以前的工作已经清楚地确定了YODA（YDA）细胞信号通路的关键作用。 YDA途径包含有丝分裂原活化蛋白（MAP）激酶模块，在受精后被激活，并促进受精卵分裂成一个大的基部和一个小的顶端子体。 这种不对称的第一次分裂定义了极性，并为随后的主轴与根和芽极的加工奠定了基础。 在该项目中，将使用多种方法分析受精卵和早期胚胎中YDA信号传导的分子机制，包括经典遗传学、比较系统发育学、分子生物学、基因组学、显微镜和生物化学。 特别注意的是致力于在受精的背景下，以及由通路和执行极性的建立为目标的基因表达程序的核心MAP激酶模块的调节因子。 预期结果将为我们理解植物的基本身体计划做出开创性贡献，并可能为培养体细胞启动胚胎的目标方法提供信息。该项目将导致培养一名博士后助理和一名研究生，涉及植物遗传学，分子生物学，基因组学和先进显微镜。 高中教师，高中生和本科生的参与是研究计划的一个组成部分;招聘将通过格鲁吉亚实习教师奖学金（GIFT）计划，赋权青年计划（EPY），大学的夏季本科生研究计划（SURP）和NSF赞助的桃州路易斯斯托克斯少数民族参与联盟（PSLSAMP）。