Arabidopsis 2010: Regulation of the proembryo boundary in Arabidopsis

拟南芥（arabidopsis）原胚边界的调控（Regulation of proembryo boundary in arabidopsis）植物体由两组干细胞组成：根和茎的顶端分生组织。这些干细胞龛是如何在早期胚胎中首先建立起来的，这在很大程度上仍然是未知的。该研究将分析预测的基因活性调节因子HAN在根干细胞生态位定位中的作用。主要通过遗传学和分子生物学的方法进行的初步工作表明，HAN决定了两种不同细胞类型之间的指导性边界的位置，称为“原胚胎边界”。当胚胎只有8个细胞时，信号就跨越了这个边界，随后形成了根干细胞生态位。一个全面的方法，包括标准的遗传和分子生物学技术，基因组学，显微镜学和生物化学，将遵循的目的是机械地了解原胚胎边界是如何建立和调节的。重点将放在分析汉汉的作用和密切相关的汉汉类因素在这个过程中。预期的结果将对我们对植物模式过程的基本理解做出重大而新颖的贡献，并可能为在培养中启动根干细胞壁龛的靶向方法提供信息。该项目将在植物遗传学、分子生物学、基因组学和高级显微镜学方面培养一名博士后和一名研究生。高中生和本科生的参与是研究计划的一个组成部分。学生将通过植物生物系的项目，以及大学的夏季本科生研究计划（SURP）和美国国家科学基金会赞助的桃州路易斯斯托克斯少数民族参与联盟（PSLSAMP）招募。

Wolfgang LukowitzProposal number IOS-0842284Arabidopsis 2010: Regulation of the proembryo boundary in ArabidopsisThe plant body is produced by two groups of stem cell, the root and shoot apical meristems. It remains largely unknown how these stem cell niches are first established in the early embryo. The proposed research will analyze the role of HAN, a predicted regulator of gene activity, in positioning the root stem cell niche. Preliminary work, largely by the methods of genetics and molecular biology, reveals that HAN determines the position of an instructive boundary between two different cell types, termed the "proembryo boundary". Signaling across this boundary, which is set up when the embryo has only eight cells, subsequently organizes the root stem cell niche.A comprehensive approach, including standard genetic and molecular biology techniques, genomics, microscopy, and biochemistry, will be followed with the aim to mechanistically understand how the proembryo boundary is established and regulated. Special emphasis will be placed on analyzing the role of HAN and closely related HAN-like factors this process. The expected results will make a significant and novel contribution to our fundamental understanding of patterning processes in plants and possibly inform targeted approaches for initiating root stem cell niches in culture.The project will lead to the training of a postdoctoral fellow and a graduate student in plant genetics, molecular biology, genomics, and advanced microscopy. Participation of high school and undergraduate students is an integral part of the research plan. Students will be recruited through programs of the Plant Biology Department, as well the University's Summer Undergraduate Research Program (SURP) and the NSF-sponsored Peach State Louis Stokes Alliance for Minority Participation (PSLSAMP).