Arabidopsis 2010: Regulation of the proembryo boundary in Arabidopsis

拟南芥2010：拟南芥原胚边界的调控

• 批准号: 0842284
• 负责人: Wolfgang Lukowitz
• 金额: $ 49.74万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0842284&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Regulation; proembryo; boundary

Wolfgang LukowitzProposal number IOS-0842284Arabidopsis 2010: Regulation of the proembryo boundary in ArabidopsisThe plant body is produced by two groups of stem cell, the root and shoot apical meristems. It remains largely unknown how these stem cell niches are first established in the early embryo. The proposed research will analyze the role of HAN, a predicted regulator of gene activity, in positioning the root stem cell niche. Preliminary work, largely by the methods of genetics and molecular biology, reveals that HAN determines the position of an instructive boundary between two different cell types, termed the "proembryo boundary". Signaling across this boundary, which is set up when the embryo has only eight cells, subsequently organizes the root stem cell niche.A comprehensive approach, including standard genetic and molecular biology techniques, genomics, microscopy, and biochemistry, will be followed with the aim to mechanistically understand how the proembryo boundary is established and regulated. Special emphasis will be placed on analyzing the role of HAN and closely related HAN-like factors this process. The expected results will make a significant and novel contribution to our fundamental understanding of patterning processes in plants and possibly inform targeted approaches for initiating root stem cell niches in culture.The project will lead to the training of a postdoctoral fellow and a graduate student in plant genetics, molecular biology, genomics, and advanced microscopy. Participation of high school and undergraduate students is an integral part of the research plan. Students will be recruited through programs of the Plant Biology Department, as well the University's Summer Undergraduate Research Program (SURP) and the NSF-sponsored Peach State Louis Stokes Alliance for Minority Participation (PSLSAMP).

Wolfgang Lukowitz建议编号IOS-0842284拟南芥2010：拟南芥原胚边界的调节植物体由两组干细胞产生，即根和芽顶端分生组织。 这些干细胞龛是如何在早期胚胎中首先建立起来的，这在很大程度上仍然是未知的。 拟议的研究将分析HAN（一种预测的基因活性调节因子）在定位根干细胞生态位中的作用。 初步的工作，主要是遗传学和分子生物学的方法，揭示了HAN确定的位置之间的两种不同的细胞类型，称为“原胚边界”的指导性的边界。当胚胎只有8个细胞时，跨越这一边界的信号传递随后组织了根干细胞nich.A综合方法，包括标准遗传和分子生物学技术，基因组学，显微镜和生物化学，将遵循旨在机械地了解原胚边界是如何建立和调节的。 特别着重分析HAN的作用以及与此密切相关的HAN样因素在这一过程中的作用。 预期的结果将为我们对植物模式化过程的基本理解做出重大而新颖的贡献，并可能为培养中启动根干细胞生态位的有针对性的方法提供信息。该项目将培养一名博士后研究员和一名研究生，涉及植物遗传学，分子生物学，基因组学和先进显微镜。 高中生和本科生的参与是研究计划的一个组成部分。 学生将通过植物生物学系的计划，以及大学的夏季本科生研究计划（SURP）和NSF赞助的桃州路易斯斯托克斯少数民族参与联盟（PSLSAMP）招募。