Metabolic multitasking: How Ralstonia solanacearum uses nitrate for plant pathogenesis

代谢多任务处理：青枯菌如何利用硝酸盐进行植物发病机制

• 批准号: 1258082
• 负责人: Caitilyn Allen
• 金额: $ 50.8万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-15 至 2017-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1258082&HistoricalAwards=false
• 关键词: Metabolic; multitasking; How; Ralstonia; solanacearum

The pathogenic bacterium Ralstonia solanacearum is very successful in the vascular tissue of its plant hosts even though this habitat contains few nutrients and little oxygen. This project's central hypothesis is that R. solanacearum uses inorganic nitrogen, one of the few abundant nutrients in plant sap, not just for food but also to generate energy and to sense and manipulate plant defenses. The investigators will use genetic and biochemical experiments to identify the specific adaptations that allow this pathogen to use inorganic nitrogen in multiple ways as it causes plant disease. Expected results include a better understanding of how R. solanacearum grows at low oxygen levels and a definition of the role(s) in virulence of nitric oxide, a common biological signal molecule that also has antimicrobial properties. R. solanacearum is a widely distributed pathogen with many hosts, so these results are likely to be relevant to diverse plant-associated microbes. Plant vascular systems are home to many microbes, ranging from destructive pathogens like R. solanacearum to valuable mutualists that can increase plant stress tolerance or growth.This project will generate basic biological knowledge about the biochemical mechanisms used by plant-inhabiting microbes. In addition, this project will contribute to the nation's scientific workforce by training a postdoctoral researcher and a doctoral student in laboratory research, teaching and mentoring, which are all key skills for future faculty. Three undergraduate students will also receive independent research experiences in microbiology.

病原细菌青枯雷尔氏菌在其植物宿主的维管组织中非常成功，即使该栖息地含有很少的营养物质和氧气。 这个项目的中心假设是R。青枯菌利用无机氮（植物汁液中为数不多的丰富营养物质之一），不仅作为食物，还产生能量，感知和操纵植物防御。研究人员将使用遗传和生化实验来确定特定的适应性，使这种病原体能够以多种方式使用无机氮，因为它会导致植物疾病。预期的结果包括更好地理解R。青枯菌在低氧水平下生长，并且确定了一氧化氮在毒性中的作用，一氧化氮是一种常见的生物信号分子，也具有抗微生物特性。R.青枯菌是一种广泛分布的病原体，具有许多宿主，因此这些结果可能与多种植物相关微生物有关。植物维管系统是许多微生物的家园，从破坏性的病原体如R。该项目将产生关于植物栖息微生物所使用的生化机制的基本生物学知识。此外，该项目将通过培训一名博士后研究员和一名博士生进行实验室研究、教学和指导，为国家的科学工作者做出贡献，这些都是未来教师的关键技能。三名本科生还将获得微生物学方面的独立研究经验。