RUI: Molecular Simulation of DNA Interactions in Oligonucleotide Microarrays

将研究10到25个碱基之间的短低聚体（包括溶液和单链结合到表面）单链和双链脱氧核糖核酸（DNA）之间的杂交和变性转变，以确定表面固定化如何影响这种平衡和链之间的相互作用。本项目的各个方面探讨了与DNA微阵列技术最相关的系统的表面和基于溶液的转换之间的差异。这些方面包括DNA分子的表面密度、分子的长度和序列的互补性。这一建议将有助于更好地确定控制双链DNA复合物形成的规则。这种双链DNA复合物的形成用于基因组学，以了解基因表达水平，也用于医学诊断和食品安全分析。

The hybridization and denaturation transitions between single- and doubled-stranded deoxyribonucleic acid (DNA) will be studied for short oligomers, between 10 and 25 bases, both in solution and with one strand bound to a surface to determine how surface immobilization affects this equilibrium and the interactions between strands. The aspects of this project explore the differences between surface- and solution-based transitions for systems that are most pertinent to DNA microarray technology. These aspects include surface density of DNA molecules, length of the molecule and complementarity of the sequence. NontechnicalThis proposal will help better determine the rules governing formation of double stranded DNA complexes. Formation of such doubled stranded DNA complexes is used in genomics to understand levels of gene expression and also in medical diagnostics and food safety analyses.