Generation and design of bifunctional bacteriophages and peptides: integrating organics and inorganics into multifunctional materials

双功能噬菌体和肽的生成和设计：将有机物和无机物整合成多功能材料

• 批准号: 210682512
• 负责人: Professor Dr. Bernhard Hauer
• 金额: --
• 依托单位: Abteilung für Technische Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/210682512?language=en
• 关键词: Generation; design; bifunctional; bacteriophages; peptides

The demand for inorganic functional materials has strongly increased over the last couple of years, with the focus now on multifunctional composite materials of different characteristics. However, the production of nanomaterials due to technical difficulties combined with the requirements for the fusion of several properties through material assembly by conventional synthesis methods is limited. The use of biomineralization to process the next generation of materials provides an innovative solution to overcome the limitations of traditional processing methods.In the proposed project it is planned to generate bioorganic-inorganic materials with electrical conductivity and thermal properties (e.g. capacitors and piezoelectrical elements). In our part we will genetically engineer bifunctional bacteriophages and peptides as scaffolds for constructing and organizing these material components. Peptides which can specifically recognize and initiate a controlled mineralization of an inorganic phase with precursor salt solutions will be identified from phage libraries and employed. Using an integrated approach based on biotechnology and bioinformatics, the molecular mechanism of peptide recognition of and binding to inorganic materials will be investigated and peptides with improved affinity and specificity will be designed.The combination of selected and engineered peptides, either as fusion proteins displayed on the phage surface or as bifunctional peptides, will facilitate the synthesis of complex multifunctional composite materials.

在过去的几年里，对无机功能材料的需求强劲增长，现在的重点是不同特性的多功能复合材料。然而，由于技术上的困难，再加上需要通过传统合成方法组装材料来融合几种性质，纳米材料的生产受到限制。利用生物矿化处理下一代材料提供了一种创新的解决方案，以克服传统处理方法的局限性。在拟议的项目中，计划生产具有导电性和热性能的生物有机-无机材料（例如电容器和压电元件）。在我们的部分，我们将基因工程双功能噬菌体和肽作为支架，用于构建和组织这些材料组件。可以特异性识别和启动无机相与前体盐溶液的受控矿化的肽将从噬菌体文库中鉴定并使用。利用生物技术和生物信息学相结合的方法，研究多肽识别和结合无机材料的分子机制，设计具有更高亲和力和特异性的多肽，将所选和工程化的多肽组合，以融合蛋白的形式展示在噬菌体表面或作为双功能肽，将有助于合成复杂的多功能复合材料。