NSF Postdoctoral Fellowship in Biology FY 2013

2013 财年 NSF 生物学博士后奖学金

• 批准号: 1306528
• 负责人: Farr Niere
• 金额: $ 25.88万
• 依托单位: NIERE FARR
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1306528&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

Defining the mechanism that regulates the insertion of the voltage-dependent potassium channel 1.1 into the surface membrane of neuronal dendrites The number of voltage-dependent potassium channel subunit 1.1 on the cell membrane tightly regulates a neuron's resting state and determines how a neuron responds to its environment accordingly. In the dendrites of neurons, voltage-dependent potassium channels that contain the 1.1 subunit influence changes in synapses that are considered to reflect the different types of learning or formation of memories. This research aims to identify the molecular mechanism governing the insertion of the voltage-dependent potassium channel subunit 1.1 into the surface membrane of dendrites. The proposed study will examine how the voltage-dependent potassium channel subunit 1.2 promotes the surface expression of subunit 1.1. Furthermore, this research will investigate the mechanism by which the suppression of the mammalian target of rapamycin, a protein that positively regulates translation, leads to the protein synthesis of the voltage-dependent potassium channel subunit 1.2. The discoveries from this study will expand the current understanding on the mechanisms that influence learning and memory at the molecular level. Furthermore, findings from this investigation will provide a holistic characterization on how the protein-the mammalian target of rapamycin-regulates the production of other proteins. Data generated from this project will be used to gain a faculty position and establish a laboratory at a university that will further elucidate the molecular events that regulate learning and memory, and support the education and research training of underrepresented populations in science.

确定调节电压依赖性钾通道1.1插入神经元树突表面膜的机制细胞膜上电压依赖性钾通道亚单位1.1的数量紧密调节神经元的静息状态，并决定神经元如何相应地对其环境做出反应。 在神经元的树突中，含有1.1亚基的电压依赖性钾通道影响突触的变化，这些变化被认为反映了不同类型的学习或记忆的形成。 本研究旨在确定电压依赖性钾通道亚基1.1插入树突表面膜的分子机制。 该研究将研究电压依赖性钾通道亚基1.2如何促进亚基1.1的表面表达。 此外，本研究还将研究抑制哺乳动物靶向雷帕霉素（一种正调节翻译的蛋白质）导致电压依赖性钾通道亚基1.2蛋白质合成的机制。这项研究的发现将扩大目前对分子水平上影响学习和记忆的机制的理解。 此外，这项研究的结果将提供一个全面的表征如何蛋白质的哺乳动物雷帕霉素的目标，调节其他蛋白质的生产。 该项目产生的数据将用于获得教师职位，并在大学建立实验室，进一步阐明调节学习和记忆的分子事件，并支持科学中代表性不足的人群的教育和研究培训。

项目成果

Analysis of Proteins That Rapidly Change Upon Mechanistic/Mammalian Target of Rapamycin Complex 1 (mTORC1) Repression Identifies Parkinson Protein 7 (PARK7) as a Novel Protein Aberrantly Expressed in Tuberous Sclerosis Complex (TSC)

对雷帕霉素复合物 1 (mTORC1) 抑制的机械/哺乳动物靶点快速变化的蛋白质进行分析，确定帕金森蛋白 7 (PARK7) 是一种在结节性硬化症复合物 (TSC) 中异常表达的新型蛋白质

• DOI: 10.1074/mcp.m115.055079
• 发表时间: 2016
• 期刊: Molecular & Cellular Proteomics
• 影响因子: 7
• 作者: Niere, Farr;Namjoshi, Sanjeev;Song, Ehwang;Dilly, Geoffrey A.;Schoenhard, Grant;Zemelman, Boris V.;Mechref, Yehia;Raab-Graham, Kimberly F.
• 通讯作者: Raab-Graham, Kimberly F.

Mammalian Target of Rapamycin (mTOR) Tagging Promotes Dendritic Branch Variability through the Capture of Ca 2+ /Calmodulin-dependent Protein Kinase II α (CaMKIIα) mRNAs by the RNA-binding Protein HuD

哺乳动物雷帕霉素靶标 (mTOR) 标记通过 RNA 结合蛋白 HuD 捕获 Ca 2 /钙调蛋白依赖性蛋白激酶 II α (CaMKIIα) mRNA 促进树突分支变异性

• DOI: 10.1074/jbc.m114.599399
• 发表时间: 2015
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Sosanya, Natasha M.;Cacheaux, Luisa P.;Workman, Emily R.;Niere, Farr;Perrone-Bizzozero, Nora I.;Raab-Graham, Kimberly F.
• 通讯作者: Raab-Graham, Kimberly F.

Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis

雷帕霉素揭示癫痫发生过程中 Kv1.1 表达的不依赖于 mTOR 的抑制

• DOI: 10.1016/j.nbd.2014.09.011
• 发表时间: 2015
• 期刊: Neurobiology of Disease
• 影响因子: 6.1
• 作者: Sosanya, Natasha M.;Brager, Darrin H.;Wolfe, Sarah;Niere, Farr;Raab-Graham, Kimberly F.
• 通讯作者: Raab-Graham, Kimberly F.