Role of peptides for cellular differentiation and host interaction of N. punctiforme

肽在点状猪笼草细胞分化和宿主相互作用中的作用

• 批准号: 211281368
• 负责人: Professorin Dr. Elke Dittmann-Thünemann
• 金额: --
• 依托单位: Arbeitsgruppe Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/211281368?language=en
• 关键词: Role; peptides; cellular; differentiation; host

The strain Nostoc punctiforme is a filamentous, nitrogen-fixing cyanobacterium, whose vegetative cells can mature in four different directions. N. punctiforme has a very broad symbiotic capacity and interacts with diverse plant species. The strain ATCC29133 is capable of producing a variety of secondary metabolites, including some of the nonribosomal peptide and polyketide classes. Our preliminary investigations suggest a role of different metabolites in the orchestration of cellular differentiation events and communication with symbiotic hosts. In the proposed project we intend to specifically look at two types of peptides: nostopeptolide(s) and anabaenopeptin. We aim to construct mutants and characterize those using microscopy, HPLC and growth curves. We will specifically look at the cellular differentiation cycle under diazotrophic conditions. We will also try to complement phenotypic effects with isolated compounds. We intend to compare the symbiotic capacity of wild type and mutant strains with different symbiotic hosts. Finally, we are planning to visualize the temporal and spatial distribution of nostopeptolide and anabaenopeptin in different cell types, differentiation steps and during symbiosis using MALDI imaging. The results of the proposed project can shed some light on the communication processes in the exceptional life cycle of N. punctiforme and are potentially interesting for scientists working on symbiosis, communication, differentiation and natural products in microorganisms.

点状念珠藻菌株是一种丝状固氮蓝细菌，其营养细胞可以在四个不同的方向上成熟。N. punctiforme具有非常广泛的共生能力，并与不同的植物物种相互作用。菌株ATCC 29133能够产生多种次级代谢产物，包括一些非核糖体肽和聚酮化合物类。我们的初步调查表明，不同的代谢产物在协调细胞分化事件和与共生宿主的通信中发挥作用。在拟议的项目中，我们打算专门研究两种类型的肽：nostopeptolide（s）和anabaenopeptin。我们的目标是构建突变体，并使用显微镜，HPLC和生长曲线的特点。我们将专门研究固氮条件下的细胞分化周期。我们还将尝试用分离的化合物来补充表型效应。我们打算比较野生型和突变株与不同共生宿主的共生能力。最后，我们计划使用MALDI成像可视化不同细胞类型，分化步骤和共生过程中的诺斯托内酯和鱼腥藻毒素的时空分布。本研究的结果可为了解N. punctiforme和潜在的兴趣，科学家的共生，通信，分化和天然产物的微生物工作。

项目成果

Nostopeptolide plays a governing role during cellular differentiation of the symbiotic cyanobacterium Nostoc punctiforme

• DOI: 10.1073/pnas.1419543112
• 发表时间: 2015-02-10
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Liaimer, Anton;Helfrich, Eric J. N.;Dittmann, Elke
• 通讯作者: Dittmann, Elke

Protective tunicate endosymbiont with extreme genome reduction.

基因组极度减少的保护性被囊动物内共生体

• DOI: 10.1111/1462-2920.12941
• 发表时间: 2015
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: I. Kehr;Dittmann
• 通讯作者: Dittmann