SBIR Phase I: Gas-Phase Two-Dimensional Electrophoresis

SBIR 第一阶段：气相二维电泳

• 批准号: 1315508
• 负责人: Anthony Midey
• 金额: $ 15万
• 依托单位: Excellims Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1315508&HistoricalAwards=false
• 关键词: SBIR; Phase; Gas; Two; Dimensional

This Small Business Innovation Research (SBIR) Phase I project will develop a prototype gas-phase two-dimensional electrophoresis (GP2DE) instrument for analyzing protein structures in mixtures with less sample and higher throughput compared to traditional two-dimensional gel electrophoresis (2DGE). The instrument leverages the previously developed multi-dimensional ion mobility spectrometry (MDIMS) method for separating gas-phase protein ions based on molecular size and shape in both time and space with high-performance IMS. Understanding proteins' functions will be crucial to achieving the advances required for personalized medicine, which will be a strong driver of the proteomics field both scientifically and economically. Consequently, designing specialized therapies requires intricate knowledge of the complete protein structure. GP2DE will provide a rapid tool for detecting differences in protein structures using coupled ion mobility spectrometry analyses to give 2D gel electrophoresis-type separations in seconds to minutes. Increasing the throughput for analyses of protein structures addresses the inefficiencies in the labor intensive 2DGE process to build the structure knowledge base more rapidly. In particular, tools that can detect post-translational modifications of proteins, such as glycosylation or phosphorylation, will be valuable to proteomics. GP2DE can detect these structural changes easily because the structural differences will result in different ion mobilities. The broader impact/commercial potential of this project, if successful, will provide a flexible, general purpose biomolecule analysis tool for researchers with limited budgets and/or facility space. This would be particularly useful for undergraduate laboratories where the shortened learning curve would allow for more productive research under typical time and resource constraints. The proposed gas-phase two-dimensional electrophoresis (G2DPE) evolves from advanced IMS technologies for structural size and shape analyses that are geared to be user-friendly. The market for proteomics research products has been projected to be several billion dollars in the next five years. GP2DE reduces the need to perform many more time consuming 2DGE analyses that have higher operating costs from labor and supplies, decreasing research costs and adding to laboratory productivity. Therefore, having another powerful tool for elucidating protein structural information will propel the rate of development of the next-generation of biotherapies better tailored to individual physiologies. The research conducted will be disseminated in peer-reviewed scientific journals and at scientific conferences to foster the adoption and advancement of multi-dimensional IMS methods for proteomics.

这项小企业创新研究（SBIR）第一阶段项目将开发一种原型气相二维电泳（GP2DE）仪器，用于分析混合物中的蛋白质结构，与传统的二维凝胶电泳（2DGE）相比，该仪器的样本量更少，通量更高。该仪器利用先前开发的多维离子迁移谱（MDIMS）方法，基于分子大小和形状在时间和空间上与高性能IMS分离气相蛋白质离子。了解蛋白质的功能对于实现个性化医疗所需的进步至关重要，这将是蛋白质组学领域在科学和经济上的强大推动力。因此，设计专门的治疗方法需要复杂的完整蛋白质结构知识。GP2DE将提供一种快速的工具，用于检测蛋白质结构的差异，使用耦合离子迁移率光谱分析，在几秒到几分钟内提供2D凝胶电泳型分离。提高蛋白质结构分析的吞吐量解决了劳动密集型2DGE过程中的低效率问题，从而更快地建立结构知识库。特别是，可以检测蛋白质翻译后修饰的工具，如糖基化或磷酸化，将对蛋白质组学有价值。GP2DE可以很容易地检测到这些结构变化，因为结构差异会导致不同的离子迁移率。该项目的广泛影响和商业潜力，如果成功，将为预算有限和/或设施空间有限的研究人员提供灵活的通用生物分子分析工具。这对本科生实验室特别有用，因为缩短的学习曲线将允许在典型的时间和资源限制下进行更富有成效的研究。拟议的气相二维电泳（G2DPE）是从先进的IMS技术发展而来的，用于结构尺寸和形状分析，旨在方便用户。蛋白质组学研究产品的市场预计在未来五年内将达到数十亿美元。GP2DE减少了执行更多耗时的2DGE分析的需要，这些分析在劳动力和供应方面具有更高的运营成本，降低了研究成本并提高了实验室生产力。因此，有了另一个强大的工具来阐明蛋白质结构信息，将推动下一代生物疗法的发展速度，更好地适应个体生理。所进行的研究将在同行评议的科学期刊和科学会议上传播，以促进采用和推进蛋白质组学的多维IMS方法。