SBIR Phase I: Novel Attractants to Control the Spotted Wing Fruit Fly, Drosophila suzukii

这个小型企业创新研究(SBIR)第一阶段项目建议为斑点翅果蝇开发新的引诱剂。铃木是东南亚特有的一种入侵害虫，以多种重要的软果类作物为目标，对美国农业构成严重威胁。害虫的范围正在迅速扩大，传统的控制方法，如农药处理，是有问题的。有效的诱杀装置可以避免大范围杀虫剂的广泛传播，保护该地区的环境、有益物种和有机农场，从而提供特定的优势。然而，这一装置取决于有效引诱剂的可用性，这是本提案的重点。该项目结合了几项新的平台技术，包括一个高通量测试系统，用于快速识别与昆虫化学感觉蛋白相互作用的分子。这些分子有可能影响铃木丝虫的行为，一旦确定，它们将随后被评估对活着的苍蝇的行为影响。因此，该项目将为下一代有效的铃木毒蛾引诱剂配方制造出可用于诱捕和种群控制的先导分子。该项目的更广泛的影响/商业潜力，如果成功，将是开发一种平台技术，适用于快速产生具有经济、农业或医药意义的害虫物种的新型防治产品。目前尚不存在特定的铃木疫霉引诱剂；铃木疫霉的诱杀装置将允许从广泛喷洒杀虫剂转向利用高效诱杀站的更有针对性、更负责任的控制方法。一种使用有效引诱剂的装置将使美国农业受益，因为它将允许安全有效地控制铃木疫霉。此外，这里使用的平台技术具有更广泛的商业影响，因为它们直接适用于其他害虫物种的控制。

This Small Business Innovation Research (SBIR) Phase I project proposes to develop novel attractants for the spotted wing fruit fly, Drosophila suzukii. D. suzukii is an invasive insect pest endemic to South East Asia that targets a variety of economically important soft-fruit crops, thus posing a serious threat to US agriculture. The pest's range is rapidly expanding and traditional control methods, such as pesticide treatment, are problematic. An effective bait-and-kill device would offer specific advantages by avoiding the wide dispersal of broad-range insecticides and protecting the environment, beneficial species, and organic farms in the area. However, this device depends on the availability of effective attractants, the focus of this proposal. This project incorporates several novel platform technologies comprising a high throughput assay system for rapidly identifying molecules that interact with insect chemosensory proteins. These molecules have the potential to affect D. suzukii behavior, and, once identified, they will be subsequently evaluated for behavioral effects on living flies. Thus, the project will result in lead molecules for a next-generation, effective D. suzukii attractant formulation useful in trapping and population control. The broader impact/commercial potential of this project, if successful, will be the development of a platform technology applicable to rapidly generating novel control products for insect pest species of economic, agricultural, or medicinal significance. No specific D. suzukii attractant currently exists; bait-and-kill devices for D. suzukii would allow a shift away from the widespread spraying of insecticides to a more targeted, responsible control methodology utilizing efficient bait-and-kill stations. A device utilizing an effective attractant would benefit the US agriculture industry since it would allow safe and effective D. suzukii control. Moreover, the platform technologies utilized herein have broader commercial impacts, as they are directly applicable to the control of other insect pest species.