SBIR Phase II: Rapid Detection of Fecal Contamination in Drinking Water

SBIR 第二阶段：快速检测饮用水中的粪便污染

• 批准号: 1058580
• 负责人: Daniel Woods
• 金额: $ 49.9万
• 依托单位: Inscent, Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-15 至 2013-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1058580&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Rapid; Detection

This Small Business Innovation Research (SBIR) Phase II project concerns a novel, rapid and costeffective detection system for fecal contamination in water supplies. Although existing methods candetect fecal contamination in water samples, improvements are needed in sensitivity, accuracy, and speed.This proposal describes the refinement of a novel sensor utilizing an insect chemosensory protein as therecognition element in a product that detects indole, a characteristic metabolite of coliform bacteria. Thebiosensor acts with high specificity and sensitivity, allowing the rapid detection of low level E. coli orfecal contamination in water supplies, and encompasses a novel implementation of lateral flowtechnology that can be used at home or in industry.The broader impacts of this research are that insect CSP-based biosensors as described constitute aplatform technology with direct applications in the detection of environmental, chemical, or biologicalcompounds or contaminants, including the detection of harmful volatile organic compounds (VOCs),quality control of foods and pharmaceuticals, the detection of toxins or stereoisomers generated duringchemical or pharmaceutical synthesis, and the detection of volatile compounds present in weapons orexplosives. These biosensors can also be used in medical diagnostics as well as numerous otherapplications where high speed, sensitivity and analyte selectivity are required. The platform technologyhas immediate application to a variety of important sensor and detector implementations that affectnumerous industries, public safety, and public health. The water safety monitor described herein is onlyone example of the applications possible.

这个小型企业创新研究（SBIR）二期项目涉及一种新的、快速的、具有成本效益的供水中粪便污染检测系统。虽然现有的方法可以检测水样中的粪便污染，但在灵敏度、准确性和速度方面需要改进。该提案描述了一种新型传感器的改进，该传感器利用昆虫化学感觉蛋白作为识别元件，用于检测大肠菌群细菌的特征代谢物吲哚。该生物传感器具有高特异性和灵敏度，可以快速检测供水中低水平的大肠杆菌排泄物污染，并包含一种新的横向流动技术，可在家庭或工业中使用。本研究的更广泛影响是，所述基于昆虫csp的生物传感器构成了一种平台技术，可直接应用于环境、化学或生物化合物或污染物的检测，包括有害挥发性有机化合物（voc）的检测、食品和药品的质量控制、化学或药物合成过程中产生的毒素或立体异构体的检测。以及对武器或炸药中挥发性化合物的检测。这些生物传感器还可用于医疗诊断以及许多其他应用，其中需要高速，灵敏度和分析物选择性。该平台技术可立即应用于影响众多行业、公共安全和公共健康的各种重要传感器和探测器实现。这里描述的水安全监测器只是可能应用的一个例子。