FSML: Enabling the next generation of cell analysis at Horn Point Laboratory

马里兰大学环境科学中心获得了一笔拨款，用于在角点实验室（http://www.umces.edu/hpl）建立一个细胞成像和分析中心，以支持浮游植物和浮游细菌的生态学和生理学研究和培训。微生物的可视化和计数是微生物生态学研究和发现的基础。HPL将更新和扩大其计数和可视化微生物细胞的能力，以支持和加强现有的分子生物学和微生物生态学设施。资金将用于购置一台台式高通量流式细胞仪、一台倒置光显微镜和一台带有现代成像系统的荧光显微镜。该设备将建立高通量培养实验和单细胞底物摄取分析的能力，并允许HPL为来自世界各地的研究生和高级本科生提供浮游植物生理学的冬季强化课程。细胞的美丽图像是微生物生态学家交流和传播他们的研究并吸引公众的一些最重要的工具。细胞分析中心将成为HPL研究生教育的一个组成部分，它将对整个切萨皮克湾和中大西洋湾的研究社区开放。夏季本科生将使用这些设备作为他们研究项目的一部分，冬季学期浮游植物生理学和生态学短期课程的学生也将使用这些设备，使HPL能够在浮游植物生理学和鉴定方面培养下一代微生物生态学家。细胞染色和计数方案将通过新建立的细胞分析中心网站提供，确保我们的发现得到广泛传播。

The University of Maryland Center for Environmental Sciences is awarded a grant to establish a cell imaging and analysis center at the Horn Point Laboratory (http://www.umces.edu/hpl) to support research and training on the ecology and physiology of phytoplankton and bacterioplankton. Visualizing and counting microorganisms are fundamental to microbial ecology research and discovery. HPL will update and expand its capacity to count and visualize microbial cells to support and enhance existing molecular biology and microbial ecology facilities. Funds will be used to acquire a benchtop high-throughput flow cytometer, an inverted light microscope, and an epifluorescent microscope with a modern imaging system. This equipment will build the capacity for high-throughput cultivation experiments and single-cell substrate uptake analysis using microautoradiography and allow HPL to offer an intensive winter-term course on phytoplankton physiology for graduate and advanced undergraduate students from around the world.Beautiful images of cells are some of the most important tools microbial ecologists have to communicate and disseminate their research and engage the general public. The cell analysis center will become an integral part of graduate education at HPL, where it will be available to the entire Chesapeake Bay and Mid-Atlantic Bight research community. Summer undergraduate students will use the equipment as part of their research projects, as will students enrolled in a winter term short course on phytoplankton physiology and ecology, allowing HPL to train the next generation of microbial ecologists in phytoplankton physiology and identification. Cell staining and counting protocols will be made available via a newly constructed website for the cell analysis center, ensuring broad dissemination of our findings.