Urban Microbial Community Dynamics: A Classroom Approach

城市微生物群落动态:课堂教学法

基本信息

  • 批准号:
    1323225
  • 负责人:
  • 金额:
    $ 53.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-08-15 至 2019-07-31
  • 项目状态:
    已结题

项目摘要

The project expands on a TUES 1 supported project, Authentic Research Experiences in Microbiology (AREM), a classroom laboratory approach, which showed that students involved in a research-based microbiology laboratory course (compared to those in a traditional laboratory course) are less likely to fail the course, show increased confidence in interpreting data, a greater appreciation for scientific research, and are more likely to advance to graduate/professional training. AREM has been institutionalized at Brooklyn College and over 400 students have participated. With this next phase of AREM, faculty from ten 4-yr colleges and four 2-yr colleges of CUNY have committed to introducing similar projects into their introductory biology laboratory courses. The original project engaged undergraduates in studying the urban microbial community. Basic protocols for determining the types of bacteria present in a swab sample from an urban site such as a subway station have been established. Procedures for analysis, including PCR amplification and sequencing of the 16S rRNA gene, have been established. Funds support faculty training workshops (including technical support for new faculty and inter-campus communication), protocol development and data display. Bioinformatics training is being facilitated by Brad Goodner through the MGAN program, with potential follow-up using Genome Solver, facilitated by Anne Rosenwald; this strategy leverages existing resources. A postdoctoral associate with experience in genomics and bioinformatics is working with students and faculty, developing their understandings of the advantages and limitations of the processes used and helping to insure: rigor in gathering and preparing samples and high-quality analysis of the data generated. An assessment plan has been developed by outside evaluators to measure the efficacy of the research experiences as a way to improve critical thinking and problem solving skills is included in the proposal. Intellectual Merit: While there is wide spread agreement on the importance of engaging undergraduates in research, it is not easy to identify projects that can be utilized with large numbers of students. With TUES-I support, the PI has demonstrated that genomic/metagenomic investigation of the urban microbiome is a research project that can be used successfully. By recruiting his colleagues at other CUNY campuses to join in, the PI is in position to transform the biology major system-wide to one that incorporates research thinking. Similar course adoptions could follow nation-wide, analogous to the successful dissemination of the HHMI sponsored and supported Hatfull Phage Hunters project and others of this type.Broader Impact: The City University of New York System has a high percentage of students from groups currrently underrepresented in the STEM workforce that would benefit from participation in this program. The PIs are actively engaged in dissemination through participation in ASMCUE, CUR, CUREnet, Genome Solver and GCAT-SEEK. A STEM Support Network is also planned to include faculty and students to share research and exchange ideas.This project is being funded jointly by the Directorate for Biological Sciences and the Directorate of Education and Human Resources, Division of Undergraduate Education as part of their efforts to support Vision and Change in Undergraduate Biology Education.
该项目扩展了TUES 1支持的项目“微生物学的真实研究经验”(AREM),这是一种课堂实验室方法,表明参与研究型微生物学实验室课程的学生(与传统实验室课程的学生相比)不太可能不及格,在解释数据方面表现出更大的信心,对科学研究有更大的欣赏,并且更有可能进入研究生/专业培训。AREM已在布鲁克林学院制度化,有400多名学生参加。在AREM的下一阶段,来自纽约市立大学十所四年制学院和四所两年制学院的教师已经承诺将类似的项目引入他们的入门生物学实验课程。最初的项目是让本科生研究城市微生物群落。已经建立了确定从城市站点(如地铁站)提取的棉签样本中存在的细菌类型的基本方案。分析程序,包括PCR扩增和16S rRNA基因测序,已经建立。资金支持教师培训研讨会(包括对新教师和校园间通信的技术支持)、协议开发和数据显示。生物信息学培训由Brad Goodner通过MGAN项目提供,后续可能使用Genome Solver,由Anne Rosenwald提供;这一策略利用了现有资源。一位具有基因组学和生物信息学经验的博士后正在与学生和教师合作,发展他们对所使用过程的优点和局限性的理解,并帮助确保:严格收集和准备样本,并对生成的数据进行高质量的分析。外部评估人员制定了一项评估计划,以衡量研究经验的有效性,作为提高批判性思维和解决问题能力的一种方式,该计划包括在提案中。智力优势:虽然人们普遍认同让本科生参与研究的重要性,但要确定一个能被大量学生利用的项目并不容易。在TUES-I的支持下,PI已经证明了城市微生物组的基因组/宏基因组研究是一个可以成功使用的研究项目。通过招募他在纽约市立大学其他校区的同事加入进来,PI有能力将整个系统的生物学专业转变为一个融合研究思维的专业。类似的课程可以在全国范围内采用,类似于HHMI赞助和支持的Hatfull噬菌体猎人项目和其他此类项目的成功传播。更广泛的影响:纽约城市大学系统中有很高比例的学生来自目前在STEM劳动力中代表性不足的群体,他们将从参与该计划中受益。通过参与ASMCUE、CUR、CUREnet、Genome Solver和GCAT-SEEK, pi积极参与传播。还计划建立一个STEM支持网络,让教师和学生分享研究成果和交流想法。该项目由生物科学理事会和本科教育司教育和人力资源理事会共同资助,作为其支持本科生物学教育的愿景和变革的努力的一部分。

项目成果

期刊论文数量(0)
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Theodore Muth其他文献

24th Annual ASM Conference for Undergraduate Educators (ASMCUE) Late-Breaking Abstracts
第 24 届 ASM 本科教育工作者年会 (ASMCUE) 最新摘要
  • DOI:
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    1.9
  • 作者:
    Heather M. Seitz;Rachel E. A. Horak;M. W. Howard;Lucy W. Kluckhohn Jones;Theodore Muth;Christopher T. Parker;Andrea Rediske;Maureen M. Whitehurst
  • 通讯作者:
    Maureen M. Whitehurst
Just the facts: calcium administration in trauma patients receiving massive blood transfusions
  • DOI:
    10.1007/s43678-025-00994-3
  • 发表时间:
    2025-07-25
  • 期刊:
  • 影响因子:
    2.000
  • 作者:
    Theodore Muth;Ian Ball;Raquel Oleksin;Alyssa Ball
  • 通讯作者:
    Alyssa Ball

Theodore Muth的其他文献

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{{ truncateString('Theodore Muth', 18)}}的其他基金

RCN-UBE: A National Network for Integrating the Study of Microbiomes into Course-based Undergraduate Research Experiences
RCN-UBE:将微生物组研究整合到基于课程的本科生研究经验的国家网络
  • 批准号:
    1827035
  • 财政年份:
    2018
  • 资助金额:
    $ 53.88万
  • 项目类别:
    Standard Grant
Authentic Research Experience in Microbiology
真实的微生物学研究经验
  • 批准号:
    0633490
  • 财政年份:
    2007
  • 资助金额:
    $ 53.88万
  • 项目类别:
    Standard Grant

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  • 批准号:
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