RUI: Specificity of Arf-dependent Golgi trafficking: A mechanistic analysis

细胞内膜泡的调节运动是生命所必需的过程。这种膜泡的运输调节了蛋白质在细胞内的运输和某些选定蛋白质从细胞中的分泌。这些过程中的微小缺陷可能会导致毁灭性的生理后果。囊泡的正确运输需要高度的特异性，因为只包含正确货物(例如特定蛋白质)的运输囊泡必须形成在适当的供体膜上，并被输送到正确的靶膜。然而，膜转运的特异性背后的机制在很大程度上仍然不清楚。在这个项目中，裂解酵母将被用来研究这些调节机制，特别是那些由ADP核糖化因子鸟嘌呤核苷酸交换因子介导的调节机制，这些因子已知在决定分泌流量的特异性方面发挥作用。更广泛的影响。该项目将通过1)融入探究式教学实验室，2)每年为4-7名高中生物学生提供研究项目，以及3)为本科生提供暑期研究机会，来加强本科生的研究。

The regulated movement of membrane vesicles within the cell is a process essential to life. This trafficking of membrane vesicles mediates the transport of proteins within the cell and the secretion of certain selected proteins from the cell. Minor defects in these processes can result in devastating physiological consequences. The correct trafficking of vesicles requires a high level of specificity, as transport vesicles containing only the correct cargoes (e.g. specific proteins) must form at the proper donor membrane and be delivered to the right target membrane. However, the mechanisms that underlie the specificity of membrane transport remain largely undefined. In this project fission yeast will be used to investigate these regulatory mechanisms, especially those mediated by the ADP Ribosylation Factor Guanine Nucleotide Exchange Factors that are known to play a role in determining the specificity of secretory traffic. Broader Impacts. This project will enhance undergraduate research through 1) integration into inquiry-based teaching laboratories, 2) providing research projects for 4-7 senior biology students each year and 3) summer research opportunities for undergraduate students.