Causes and Consequences of Variation in cyclic AMP - Protein Kinase A Signaling in Yeast

酵母中环 AMP - 蛋白激酶 A 信号传导变异的原因和后果

• 批准号: 1330545
• 负责人: Paul Magwene
• 金额: $ 30万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330545&HistoricalAwards=false
• 关键词: Causes; Consequences; Variation; cyclic; AMP

Few detailed studies have been conducted on the influence of genetic variation on signal transduction pathways, even though knowledge of this is important to the understanding of cell behavior. The cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signaling pathway is conserved in animals and fungi and regulates cellular growth and differentiation. For example, in yeast (Saccharomyces cerevisiae) cAMP-PKA signaling plays a central role in regulating developmental transitions such as the shift from typical yeast form growth to filamentous growth and the formation of biofilms. The goal of this project is to develop and use fluorescence resonance energy transfer (FRET)-based sensors to characterize how signaling in the yeast cAMP-PKA pathway varies with genetic background and environmental stimuli, and the manner in which this affects cellular growth and differentiation. Broader Impacts. This project will support the training and mentoring of postdoctoral, undergraduate and high school trainees. It will also support a continuing outreach project lead by the PI that provides a week-long course on microbiology and systems biology to advanced high school students. The PI will use materials from this outreach course to develop on-line science investigation modules that will be made available to high school science educators at the state and national level.

很少有关于遗传变异对信号转导途径的影响的详细研究，尽管这方面的知识对理解细胞行为很重要。环腺苷单磷酸蛋白激酶A （cAMP-PKA）信号通路在动物和真菌中是保守的，并调节细胞生长和分化。例如，在酵母（Saccharomyces cerevisiae）中，cAMP-PKA信号在调节发育转变（如从典型的酵母形态生长转变为丝状生长和生物膜的形成）中起着核心作用。本项目的目标是开发和使用基于荧光共振能量转移（FRET）的传感器来表征酵母cAMP-PKA通路中的信号如何随遗传背景和环境刺激而变化，以及这影响细胞生长和分化的方式。更广泛的影响。本项目将支持博士后、本科生和高中学员的培训和指导。它还将支持PI领导的一个持续的外展项目，该项目为高级高中学生提供为期一周的微生物学和系统生物学课程。PI将使用这门拓展课程的材料开发在线科学调查模块，这些模块将提供给州和国家层面的高中科学教育者。