Impact of Alternative Substrates on Methane, Methanol, and Chloromethane Metabolism of Methylotrophs in Forest Soils

替代基质对森林土壤中甲基营养菌的甲烷、甲醇和氯甲烷代谢的影响

• 批准号: 212127456
• 负责人: Professor Dr. Steffen Kolb
• 金额: --
• 依托单位: Lehrstuhl für Ökologische Mikrobiologie (ÖMIK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/212127456?language=en
• 关键词: Impact; Alternative; Substrates; Methane; Methanol

Methylotrophic microbes in forest soils govern to a substantial degree the global exchange of methane, methanol, and chloromethane of terrestrial ecosystems with the atmosphere. Methane is a greenhouse gas. Methanol and chloromethane affect ozone formation. Forest soil methylotrophs utilise these compounds as energy and carbon sources. However, ambient concentrations of methane, methanol, and chloromethane are such low that it is unlikely that their utilisation allows for sufficient energy conservation of methylotrophs. Thus, forest soil methylotrophs likely utilise alternative substrates that are abundant in forest soil, such as hydrolysis products of cellulose and hemicellulose, anaerobic degradation products of organic matter, nalkanes from plant waxes, and soluble metabolites of lignin. The proposed project will resolve trophic strategies of methylotrophs that utilise one-carbon atmospheric gases, and thrive in forest soils. Soil samples of two temperate beech stands will be investigated (Steigerwald, Solling). The proposed experiments will allow for identification of active methylotrophs and their metabolic pathways at in situ-relevant substrate concentrations using state of the art stable isotope probing techniques, cultivation, metatranscriptomics and –proteomics, and substrate consumption kinetics. Furthermore, experiments will reveal insights into the impact of soil microbial communities on the yet insufficiently resolved global cycles of methanol and chloromethane.

森林土壤中的甲基营养微生物在很大程度上控制着陆地生态系统中甲烷、甲醇和氯甲烷与大气的全球交换。甲烷是一种温室气体。甲醇和氯甲烷影响臭氧的形成。森林土壤甲基营养菌利用这些化合物作为能源和碳源。然而，甲烷，甲醇和氯甲烷的环境浓度是如此之低，它是不可能的，它们的利用允许足够的能量保存的甲基营养。因此，森林土壤甲基营养菌可能利用森林土壤中丰富的替代底物，如纤维素和半纤维素的水解产物、有机物质的厌氧降解产物、来自植物蜡的正烷烃和木质素的可溶性代谢产物。拟议的项目将解决利用一碳大气气体的甲基营养生物的营养策略，并在森林土壤中茁壮成长。将对两个温带山毛榉林的土壤样品进行调查（Steigerwald，索林）。拟议的实验将允许使用最先进的稳定同位素探测技术，培养，元转录组学和蛋白质组学，和底物消耗动力学的活性甲基营养菌和它们的代谢途径在原位相关的底物浓度的识别。此外，实验将揭示土壤微生物群落对尚未充分解决的甲醇和氯甲烷全球循环的影响。