Understanding and Modulation of Interfacial Properties within Plant Cell Wall Pores to Facilitate Enzymatic Deconstruction and Conversion to Biofuels

了解和调节植物细胞壁孔内的界面特性以促进酶解构和转化为生物燃料

• 批准号: 1336622
• 负责人: David Hodge
• 金额: $ 29.99万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1336622&HistoricalAwards=false
• 关键词: Understanding; Modulation; Interfacial; Properties; within

PI: Hodge, DavidProposal Number: 1336622Institution: Michigan State UniversityTitle: Understanding and Modulation of Interfacial Properties within Plant Cell Wall Pores to Facilitate Enzymatic Deconstruction and Conversion to BiofuelsThis project will investigate the multifaceted role that the non-covalent forces exerted by plant cell wall polymers within nanoscale cell wall pores have on a number of important phenomena influencing plant cell wall deconstruction for biofuel production, how these forces are impacted by plant cell wall properties and how these properties evolve during pretreatment and hydrolysis that lead to outcomes of improved polysaccharide conversion. Specifically this involves the role of surface properties in influencing: (1) water infiltration into the cell wall and cell wall swelling, (2) cellulolytic enzyme accessibility to binding sites within the cell wall, and (3) enzyme binding to chemically and physically modified surfaces within the cell wall. For this, the PIs will investigate a new paradigm for understanding plant cell wall recalcitrance, specifically that the limitations of cell wall porosity and inaccessible surface area to enzymes, can be best investigated in the context of understanding the plant cell wall matrix as a water-swellable hydrogel and understanding the porosity in the context of hydrogel swelling due to the opposing forces of water swelling versus the resistance of the plant cell wall matrix to swelling imparted by lignification. Additionally, the PIs propose that using a wider range of plant cell wall sources than have been explored in the past (graminaceous monocots and a woody dicot) and exploring a wider range of pretreatment/delignification conditions that result in a diverse range of cell wall properties (lignin contents, carboxylate contents) they will be able to significantly improve the understanding of how cell wall matrix properties impact water swelling and porosity and be able to link these differences to improved enzymatic conversion.By comparing the diverse cell walls with the diverse properties, this project will address several important outstanding problems in cellulose hydrolysis: (1) untangle the complex relationship between cell wall rigidity, lignin content, cell wall swelling, and porosity in its impact on cell wall enzymatic digestibility, (2) understand the limiting factors controlling the penetration of charged and uncharged sets of polymer probes, enzymes, and non-catalytic glycan-binding protein modules into cell wall pores, and (3) develop an improved understanding of the fundamental differences in properties influencing recalcitrance in grasses versus woody dicots.The project work will help enable the development of bioenergy technologies for displacing imported petroleum, and providing rural income and employment. The education of undergraduate engineering students by engaging them with hands-on experiences and examples of these bioenergy technologies is a logical development in the evolution of engineering education. The PIs will further develop an international bioenergy education program with the goal of enhancing student knowledge acquisition and increasing self-directed and life-long learning through the diverse experiences and perspectives that students gain in this international environment.

主要研究者：Hodge，David提案编号：1336622机构：密歇根州立大学职称：了解和调节植物细胞壁孔内的界面性质，以促进酶促解构和转化为生物燃料本项目将研究植物细胞壁聚合物在纳米级细胞壁孔内施加的非共价力对影响植物细胞壁解构用于生物燃料生产的许多重要现象的多方面作用，这些力如何受到植物细胞壁性质的影响，以及这些性质在预处理和水解过程中如何演变，从而导致改进的多糖转化的结果。具体而言，这涉及表面性质在影响以下方面的作用：（1）水渗入细胞壁和细胞壁溶胀，（2）纤维素分解酶对细胞壁内结合位点的可及性，以及（3）酶与细胞壁内化学和物理改性表面的结合。为此，PI将研究一种新的范式，用于理解植物细胞壁的多孔性，特别是细胞壁孔隙度和酶不可接近的表面积的限制，可以在理解植物细胞壁基质为水的背景下进行最好的研究，可溶胀的水凝胶和理解由于水溶胀的相反力与水凝胶的阻力导致的水凝胶溶胀的背景下的孔隙率。植物细胞壁基质对木质化引起的肿胀的反应。 此外，PI建议使用比过去探索的更广泛的植物细胞壁来源（禾本科单子叶植物和木本双子叶植物），并探索更广泛的预处理/脱木质素条件，从而产生多种细胞壁性质（木质素含量，羧酸盐含量）他们将能够显著提高对细胞壁基质性质如何影响水膨胀和孔隙率的理解，并能够将这些差异与改善的酶促反应联系起来。通过比较具有不同性质的不同细胞壁，该项目将解决纤维素水解中的几个重要的突出问题：（1）解开细胞壁刚性、木质素含量、细胞壁膨胀和孔隙率在其对细胞壁酶消化率的影响中的复杂关系，（2）理解控制带电和不带电聚合物探针组的渗透的限制因素，该项目的工作将有助于开发生物能源技术，以取代进口石油，并为农村提供收入和就业机会。通过让工程专业本科生参与这些生物能源技术的实践经验和实例来教育他们，是工程教育发展的一个合乎逻辑的发展。PI将进一步开发国际生物能源教育计划，目标是通过学生在国际环境中获得的各种经验和观点，提高学生的知识获取能力，并增加自我导向和终身学习。

项目成果

Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling

• DOI: 10.1039/c4ra00824c
• 发表时间: 2014-04
• 期刊: RSC Advances
• 影响因子: 3.9
• 作者: Muyang Li;S. Pattathil;M. Hahn;D. Hodge

Prediction of Cell Wall Properties and Response to Deconstruction Using Alkaline Pretreatment in Diverse Maize Genotypes Using Py-MBMS and NIR

使用 Py-MBMS 和 NIR 预测不同玉米基因型的细胞壁特性和对碱预处理解构的响应

• DOI: 10.1007/s12155-016-9798-z
• 发表时间: 2016
• 期刊: BioEnergy Research
• 影响因子: 3.6
• 作者: Li, Muyang;Williams, Daniel L.;Heckwolf, Marlies;de Leon, Natalia;Kaeppler, Shawn;Sykes, Robert W.;Hodge, David
• 通讯作者: Hodge, David

Water Sorption in Pretreated Grasses as a Predictor of Enzymatic Hydrolysis Yields

预处理草中的水吸附作为酶水解产量的预测因子

• DOI: 10.1016/j.biortech.2017.08.200
• 发表时间: 2017
• 期刊: Bioresource Technology
• 影响因子: 11.4
• 作者: Williams, Daniel L.;Crowe, Jacob D.;Ong, Rebecca G.;Hodge, David B.
• 通讯作者: Hodge, David B.

Corn stover semi-mechanistic enzymatic hydrolysis model with tight parameter confidence intervals for model-based process design and optimization

• DOI: 10.1016/j.biortech.2014.11.062
• 发表时间: 2015-02-01
• 期刊: BIORESOURCE TECHNOLOGY
• 影响因子: 11.4
• 作者: Scott, Felipe;Li, Muyang;Aroca, German
• 通讯作者: Aroca, German