MRI: Acquisition of a Synergy H1m Monochromator-Based Multi-Mode Microplate Spectrophotometer for Research and Undergraduate Research Training

这项主要研究仪器奖授予了索尔兹伯里大学，用于购买BioTek Synergy H1m微孔板分光光度计，这将促进生物科学和化学部门的研究、培训和教学。该协同H1m微孔板读取器是一个可编程的，高灵敏度的仪器，快速测量紫外-可见光吸光度，荧光，或化学发光在精确指定的波长内的大量小样品。获得这种灵活，低维护但高性能的仪器将实现超出现有技术和能力的多样品，温度控制，动态生物测定和分析。至少四名索尔兹伯里大学细胞和分子生物学、生理学和生物化学教员的研究项目将受益于这台仪器，包括研究基因对氧化应激的特异性影响、去泛素酶活性的调节、将生化功能分配给具有已知结构的未表征蛋白质，以及探索快速温度波动对代谢过程和细胞膜结构的影响。这种高容量、多功能和灵敏的仪器增强了对正在进行的研究问题的现有方法，促进了现有的合作，并将激励未来的研究项目。除了对索尔兹伯里大学的研究生产力产生重大影响外，该仪器还将有助于培养将成为下一代科学家和技术人员的学生。索尔兹伯里大学是一所中等规模的、经过地区认证的四年制综合性大学，专注于卓越的本科教育，并致力于学生的研究参与。在索尔兹伯里大学（Salisbury University）进行研究的本科生和应用生物学硕士研究生，以及他们在邻近的马里兰大学东岸分校（University of Maryland, Eastern Shore）的合作者，将熟练掌握该仪器的使用和随后的数据分析。在索尔兹伯里大学，生物科学系和化学系的学生将通过结合细胞生物学和生物化学实验课程，接触到仪器、它的能力和它的应用力量。所有参加生物化学实验课程的学生都将极大地受益于H1m微孔板阅读器在蛋白质纯化，酶动力学和蛋白质稳定性分析方面的扩展可能性。接触这种先进的技术仪器，并展示其在高通量筛选技术中的“实际”应用，将增加学生的兴奋感，培养他们对生物和化学相关领域的兴趣。

This Major Research Instrumentation award to Salisbury University for the acquisition of a BioTek Synergy H1m microplate spectrophotometer will facilitate research, training, and teaching in the Departments of Biological Sciences and Chemistry. The Synergy H1m microplate reader is a programmable, highly sensitive instrument that rapidly measures UV-visible light absorbance, fluorescence, or chemiluminescence at precisely specified wavelengths within a large number of small samples. Acquisition of this flexible, low maintenance yet high performance instrument will enable a wide range of multi-sample, temperature-controlled, kinetic bioassays and analyses that are beyond existing technology and capacity. The research projects of at least four Salisbury University faculty in cell and molecular biology, physiology, and biochemistry that will benefit from this instrument include those involving the study of gene-specific effects on oxidative stress, regulation of deubiquitinase enzyme activity, assigning biochemical functions to uncharacterized proteins with known structures, and exploring the effect of rapid temperature fluctuations on metabolic processes and cell membrane structure. This high capacity, versatile, and sensitive instrument enhances current approaches to on-going research questions, facilitates existing collaborations, and will inspire future research projects. In addition to the significant impact that the Synergy H1m microplate reader will have on Salisbury University's research productivity, the instrument will also facilitate the training of students who will become the next generation of scientists and technicians. Salisbury University is a mid-sized, regionally accredited, four-year comprehensive institution with a focus on excellence in undergraduate education and a strong commitment to student research engagement. The undergraduate research and Master of Science in Applied Biology students working with faculty at Salisbury University, and their collaborator at the neighboring University of Maryland, Eastern Shore, will become adept at the instrument's use and subsequent data analysis. At Salisbury University, students in both the Departments of Biological Sciences and Chemistry will gain exposure to the instrument, its capacities, and the power of its applications through incorporation into cell biology and biochemistry laboratory course curricula. All students enrolled in biochemistry laboratory courses will benefit immensely from the expanded possibilities in protein purification, enzyme kinetics, and protein stability analyses facilitated by the H1m microplate reader. Exposure to this advanced-technology instrument and the demonstration of its 'real-life' applications in high throughput screening techniques will increase excitement and nurture students' interest in fields related to biology and chemistry.