MRI: Acquisition of a Synergy H1m Monochromator-Based Multi-Mode Microplate Spectrophotometer for Research and Undergraduate Research Training

索尔兹伯里大学获得的BioTek Synergy H1 m微孔板分光光度计的主要研究仪器奖将促进生物科学和化学系的研究，培训和教学。 Synergy H1 m酶标仪是一种可编程的高灵敏度仪器，可快速测量大量小样本中精确指定波长的紫外-可见光吸光度、荧光或化学发光。 购买这款灵活、低维护、高性能的仪器，将能够进行多种多样的多样品、温度控制、动力学生物测定和分析，这超出了现有的技术和能力。 索尔兹伯里大学至少有四名教师在细胞和分子生物学，生理学和生物化学方面的研究项目将受益于该仪器，包括那些涉及研究基因对氧化应激的特异性影响，去泛素酶活性的调节，将生化功能分配给具有已知结构的未表征蛋白质，以及探索快速温度波动对代谢过程和细胞膜结构的影响。 这种高容量，多功能和敏感的仪器增强了当前正在进行的研究问题的方法，促进了现有的合作，并将激发未来的研究项目。 除了Synergy H1 m酶标仪将对索尔兹伯里大学的研究生产力产生重大影响外，该仪器还将促进学生的培训，他们将成为下一代科学家和技术人员。 索尔兹伯里大学是一所中等规模，区域认可的四年制综合性机构，专注于卓越的本科教育和对学生研究参与的坚定承诺。 本科研究和应用生物学硕士学生与索尔兹伯里大学的教师合作，以及他们在邻近的马里兰州东海岸大学的合作者，将熟练掌握仪器的使用和随后的数据分析。 在索尔兹伯里大学，生物科学和化学系的学生将通过纳入细胞生物学和生物化学实验室课程，接触仪器，其能力及其应用的力量。 所有参加生物化学实验室课程的学生将从H1 m酶标仪促进的蛋白质纯化，酶动力学和蛋白质稳定性分析的扩展可能性中受益匪浅。 接触这种先进的技术仪器和其在高通量筛选技术中的“现实生活”应用的演示将增加兴奋和培养学生对生物和化学相关领域的兴趣。

This Major Research Instrumentation award to Salisbury University for the acquisition of a BioTek Synergy H1m microplate spectrophotometer will facilitate research, training, and teaching in the Departments of Biological Sciences and Chemistry. The Synergy H1m microplate reader is a programmable, highly sensitive instrument that rapidly measures UV-visible light absorbance, fluorescence, or chemiluminescence at precisely specified wavelengths within a large number of small samples. Acquisition of this flexible, low maintenance yet high performance instrument will enable a wide range of multi-sample, temperature-controlled, kinetic bioassays and analyses that are beyond existing technology and capacity. The research projects of at least four Salisbury University faculty in cell and molecular biology, physiology, and biochemistry that will benefit from this instrument include those involving the study of gene-specific effects on oxidative stress, regulation of deubiquitinase enzyme activity, assigning biochemical functions to uncharacterized proteins with known structures, and exploring the effect of rapid temperature fluctuations on metabolic processes and cell membrane structure. This high capacity, versatile, and sensitive instrument enhances current approaches to on-going research questions, facilitates existing collaborations, and will inspire future research projects. In addition to the significant impact that the Synergy H1m microplate reader will have on Salisbury University's research productivity, the instrument will also facilitate the training of students who will become the next generation of scientists and technicians. Salisbury University is a mid-sized, regionally accredited, four-year comprehensive institution with a focus on excellence in undergraduate education and a strong commitment to student research engagement. The undergraduate research and Master of Science in Applied Biology students working with faculty at Salisbury University, and their collaborator at the neighboring University of Maryland, Eastern Shore, will become adept at the instrument's use and subsequent data analysis. At Salisbury University, students in both the Departments of Biological Sciences and Chemistry will gain exposure to the instrument, its capacities, and the power of its applications through incorporation into cell biology and biochemistry laboratory course curricula. All students enrolled in biochemistry laboratory courses will benefit immensely from the expanded possibilities in protein purification, enzyme kinetics, and protein stability analyses facilitated by the H1m microplate reader. Exposure to this advanced-technology instrument and the demonstration of its 'real-life' applications in high throughput screening techniques will increase excitement and nurture students' interest in fields related to biology and chemistry.