MRI: Acquisition of a Synergy H1m Monochromator-Based Multi-Mode Microplate Spectrophotometer for Research and Undergraduate Research Training

MRI：采购基于 Synergy H1m 单色仪的多模式微孔板分光光度计，用于研究和本科生研究培训

• 批准号: 1337534
• 负责人: Patti Erickson
• 金额: $ 4.89万
• 依托单位: Salisbury University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337534&HistoricalAwards=false
• 关键词: MRI; Acquisition; Synergy; H1m; Monochromator

This Major Research Instrumentation award to Salisbury University for the acquisition of a BioTek Synergy H1m microplate spectrophotometer will facilitate research, training, and teaching in the Departments of Biological Sciences and Chemistry. The Synergy H1m microplate reader is a programmable, highly sensitive instrument that rapidly measures UV-visible light absorbance, fluorescence, or chemiluminescence at precisely specified wavelengths within a large number of small samples. Acquisition of this flexible, low maintenance yet high performance instrument will enable a wide range of multi-sample, temperature-controlled, kinetic bioassays and analyses that are beyond existing technology and capacity. The research projects of at least four Salisbury University faculty in cell and molecular biology, physiology, and biochemistry that will benefit from this instrument include those involving the study of gene-specific effects on oxidative stress, regulation of deubiquitinase enzyme activity, assigning biochemical functions to uncharacterized proteins with known structures, and exploring the effect of rapid temperature fluctuations on metabolic processes and cell membrane structure. This high capacity, versatile, and sensitive instrument enhances current approaches to on-going research questions, facilitates existing collaborations, and will inspire future research projects. In addition to the significant impact that the Synergy H1m microplate reader will have on Salisbury University's research productivity, the instrument will also facilitate the training of students who will become the next generation of scientists and technicians. Salisbury University is a mid-sized, regionally accredited, four-year comprehensive institution with a focus on excellence in undergraduate education and a strong commitment to student research engagement. The undergraduate research and Master of Science in Applied Biology students working with faculty at Salisbury University, and their collaborator at the neighboring University of Maryland, Eastern Shore, will become adept at the instrument's use and subsequent data analysis. At Salisbury University, students in both the Departments of Biological Sciences and Chemistry will gain exposure to the instrument, its capacities, and the power of its applications through incorporation into cell biology and biochemistry laboratory course curricula. All students enrolled in biochemistry laboratory courses will benefit immensely from the expanded possibilities in protein purification, enzyme kinetics, and protein stability analyses facilitated by the H1m microplate reader. Exposure to this advanced-technology instrument and the demonstration of its 'real-life' applications in high throughput screening techniques will increase excitement and nurture students' interest in fields related to biology and chemistry.

索尔兹伯里大学因购买 BioTek Synergy H1m 微孔板分光光度计而获得的重大研究仪器奖将促进生物科学和化学系的研究、培训和教学。 Synergy H1m 酶标仪是一款可编程、高灵敏度仪器，可在大量小样品中以精确指定的波长快速测量紫外-可见光吸光度、荧光或化学发光。 购买这种灵活、低维护且高性能的仪器将能够实现超出现有技术和能力的广泛的多样品、温度控制、动力学生物测定和分析。 索尔兹伯里大学至少四名细胞和分子生物学、生理学和生物化学教师的研究项目将受益于该仪器，包括研究基因对氧化应激的特异性影响、去泛素酶活性的调节、为具有已知结构的未表征蛋白质分配生化功能，以及探索快速温度波动对代谢过程和细胞的影响。 膜结构。 这种高容量、多功能且灵敏的仪器增强了当前研究问题的方法，促进了现有的合作，并将激发未来的研究项目。 Synergy H1m 酶标仪除了对索尔兹伯里大学的研究生产力产生重大影响外，还将促进对学生的培训，使他们成为下一代科学家和技术人员。 索尔兹伯里大学是一所中型、地区认可的四年制综合性大学，专注于卓越的本科教育和对学生研究参与的坚定承诺。 应用生物学本科生和理学硕士学生与索尔兹伯里大学的教师以及他们在邻近的马里兰大学东海岸分校的合作者一起工作，将熟练掌握该仪器的使用和随后的数据分析。 在索尔兹伯里大学，生物科学系和化学系的学生将通过纳入细胞生物学和生物化学实验室课程，接触到该仪器、其功能及其应用的强大功能。 所有参加生物化学实验室课程的学生都将受益于 H1m 酶标仪在蛋白质纯化、酶动力学和蛋白质稳定性分析方面的扩展可能性。 接触这种先进技术仪器并展示其在高通量筛选技术中的“现实”应用将增加学生的兴奋感并培养学生对生物学和化学相关领域的兴趣。