Comparative-, Functional-, and Epi-Genomics of Legumes and Nodule Formation

PI: Scott a . Jackson (University of Georgia)CoPIs: Robert J. Schmitz （University of Georgia）和Marc Libault （University of Oklahoma-Norman）豆科植物是一组与土壤微生物相互作用以固定大气氮供植物使用的植物，是可持续农业的一个组成部分。虽然这是一个基本的过程，但有许多组成部分尚不清楚，对它们的更好理解可能会导致植物更有效地固定氮。在这个项目中，表观遗传因素，如非孟德尔，将被检查，以确定这些因素如何响应与土壤微生物的相互作用，并在植物固氮器官成熟过程中。生成的数据将包括微生物感染期间和之后的转录组，以及表观基因组，包括可能诱导或响应感染的DNA甲基化和DNA组蛋白修饰。除了对豆科植物固氮器官的发育过程有更基本的了解外，新墨西哥州大学的本科生将在学年期间参加暑期研究实习和合作研究。外展研讨会将在新墨西哥州举行，以帮助教师和学生开发基因组学和生物信息学的机会，将这些工具整合到课程中，并使他们接触到这一不断发展的领域的机会。所有序列数据将存储在NCBI Short Read Archive （SRA）中，相关元数据将存储在Gene Expression Omnibus （GEO）中。此外，本项目产生的所有表观遗传数据将通过适当的基因组数据库提供，包括Phytozome， SoyBase和Medicago.org。

PI: Scott A. Jackson (University of Georgia)CoPIs: Robert J. Schmitz (University of Georgia) and Marc Libault (University of Oklahoma-Norman)Legumes are a group of plants that interact with soil-borne microorganisms to fix atmospheric nitrogen for plant use, an integral part of sustainable agriculture. Although this is a fundamental process, there are many components that are poorly understood and for which a better understanding may lead to plants that more efficiently fix nitrogen. In this project, epigenetic factors, e.g. non-Mendelian, will be examined to determine how these factors respond to interaction with soil microbes and during maturation of the nitrogen fixing organs in the plant. Data generated will include transcriptomes during and after microbe infection as well as epigenomes including DNA methylation and DNA histone modifications that may be induced or responsive to infection.In addition to a more fundamental understanding of the process of developing organs for nitrogen fixation in legumes, undergraduate students from colleges in New Mexico will be included in summer research internships and collaborative research during the academic year. Outreach seminars will be held in New Mexico to help develop genomics and bioinformatics opportunities for teachers and students to integrate these tools into curriculum and to expose them to the opportunities in this growing field. All sequence data will be deposited at the NCBI Short Read Archive (SRA) with associated metadata deposited at the Gene Expression Omnibus (GEO). In addition, all epigenetic data generated in this project will be available through appropriate genome databases including Phytozome, SoyBase and Medicago.org.