Comparative-, Functional-, and Epi-Genomics of Legumes and Nodule Formation

PI：Scott A.杰克逊（格鲁吉亚大学）CoPI：罗伯特·J·施密茨（格鲁吉亚大学）和马克·利博特（俄克拉荷马-诺曼大学）豆科植物是一组与土壤微生物相互作用以固定大气氮供植物使用的植物，这是可持续农业的一个组成部分。虽然这是一个基本的过程，但有许多成分知之甚少，更好的理解可能会导致植物更有效地固氮。 在该项目中，将检查表观遗传因素，例如非孟德尔因素，以确定这些因素如何响应与土壤微生物的相互作用以及植物固氮器官成熟期间的反应。 产生的数据将包括转录组期间和之后的微生物感染以及表观基因组，包括DNA甲基化和DNA组蛋白修饰，可能会引起或响应infection.In的发展过程中的豆科植物固氮器官的更基本的理解，本科生从学院在新墨西哥州将被列入夏季研究实习和合作研究在学年期间。 将在新墨西哥州举行外联研讨会，以帮助教师和学生开发基因组学和生物信息学的机会，将这些工具纳入课程，并使他们接触到这个不断发展的领域的机会。 所有序列数据将存放在NCBI Short Read Archive（SRA），相关元数据存放在Gene Expression Omnibus（GEO）。 此外，该项目中产生的所有表观遗传数据将通过适当的基因组数据库提供，包括Phytozome，Medicago.org。

PI: Scott A. Jackson (University of Georgia)CoPIs: Robert J. Schmitz (University of Georgia) and Marc Libault (University of Oklahoma-Norman)Legumes are a group of plants that interact with soil-borne microorganisms to fix atmospheric nitrogen for plant use, an integral part of sustainable agriculture. Although this is a fundamental process, there are many components that are poorly understood and for which a better understanding may lead to plants that more efficiently fix nitrogen. In this project, epigenetic factors, e.g. non-Mendelian, will be examined to determine how these factors respond to interaction with soil microbes and during maturation of the nitrogen fixing organs in the plant. Data generated will include transcriptomes during and after microbe infection as well as epigenomes including DNA methylation and DNA histone modifications that may be induced or responsive to infection.In addition to a more fundamental understanding of the process of developing organs for nitrogen fixation in legumes, undergraduate students from colleges in New Mexico will be included in summer research internships and collaborative research during the academic year. Outreach seminars will be held in New Mexico to help develop genomics and bioinformatics opportunities for teachers and students to integrate these tools into curriculum and to expose them to the opportunities in this growing field. All sequence data will be deposited at the NCBI Short Read Archive (SRA) with associated metadata deposited at the Gene Expression Omnibus (GEO). In addition, all epigenetic data generated in this project will be available through appropriate genome databases including Phytozome, SoyBase and Medicago.org.